Anti-PSCA (AM1) h(DAP12) CAR, pCDCAR1 vector

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Published Data

CAR scFv data FCM

Fig.1 Binding studies for functional characterization of scFvs and scFv-BAPs via the c-myc epitope for validation of mono-biotinylated scFv(h-AM1)-BAP and scFv(MR1.1)-BAP were undertaken using fow cytometry.

CAR Construction :

Open histograms represent staining controls using only secondary antibodies.

Schau, I., Michen, S., Hagstotz, A., Janke, A., Schackert, G., Appelhans, D., & Temme, A. (2019). Targeted delivery of TLR3 agonist to tumor cells with single chain antibody fragment-conjugated nanoparticles induces type I-interferon response and apoptosis. Scientific Reports, 9(1), 3299.

CAR scFv data FCM

Fig.2 scFv(h-AM1)-BAP-RICIA-induced apoptosis in HEK-BluehTLR3/PSCA cells was analyzed via Annexin V-FITC/PI staining.

CAR Construction :

Fig.2 scFv(h-AM1)-BAP-RICIA-induced apoptosis in HEK-BluehTLR3/PSCA cells was analyzed via Annexin V-FITC/PI staining.

Data are represented as mean±s.d.

CAR scFv data FCM

Fig.3 Crosslinking of scFv(h-AM1) causes signifcant PSCA receptor internalization on HEK293TPSCA cells in a time-dependent manner.

CAR Construction :

Fig.3 Crosslinking of scFv(h-AM1) causes signifcant PSCA receptor internalization on HEK293TPSCA cells in a time-dependent manner.

Internalization of PSCA afer treatment with monovalent or crosslinked scFv(h-AM1) was assessed via fow cytometry.

CAR scFv data FCM

Fig.4 Targeted delivery of scFv (AM1)-P-BAP-guided polyplexes in PSCA-positive cells.

CAR Construction :

Fig.4 Targeted delivery of scFv (AM1)-P-BAP-guided polyplexes in PSCA-positive cells.

Data are represented as mean±s.d.

Jugel, W., Aigner, A., Michen, S., Hagstotz, A., Ewe, A., Appelhans, D., ... & Tietze, S. (2021). Targeted RNAi of BIRC5/survivin using antibody-conjugated poly (propylene imine)-based polyplexes inhibits growth of PSCA-positive tumors. Pharmaceutics, 13(5), 676.

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