CD99

The following are some CD99 CAR-related products we provide for customers to choose from. Meanwhile, you can search for more suitable products through the following search box.

Background

CD99 is a 32-kDa cell surface glycoprotein that expressed in almost all leukocyte lineage cells. CD99 participates in many important biological processes, such as cell adhesion, leukocyte migration, infiltration, and transmembrane protein transport processes. Meanwhile, it influences a variety of signaling pathways associated with cancer, inflammation, and immune response. Given the features of CD99, it is a promising therapeutic target for many diseases.

Associated Diseases

• Acute Myeloid Leukemia
• Acute Lymphoblastic Leukemia/Lymphoma
• Myelodysplastic Syndromes

Anti-CD99 CAR-T Services

CD99 CAR Design & Construction

For CAR structure design, we provide a variety of robust CAR construction, including classical CAR structures, special armed CAR designs, etc. At the same time, we are committed to customizing more suitable products for customers to meet the needs of the project.

Fig.1 Schematic diagram of CD99 CAR construction.¹

Anti-CD99 CAR-T In Vitro Assays

Anti-CD99 CAR-T Expansion Test

Cell proliferation test is a relatively routine but critical test in the CART development, we provide sensitive detection experiments through a variety of detection methods, such as the MTT test, Ki 67 detection, and cell dynamic analysis.

Fig.2 Proliferation test of anti-CD99 CAR T cells for 14 days.¹

Anti-CD99 CAR Expression Test

CAR expression detection is designed to analyze the CAR transfection and expression of CART cells, providing relevant data references for further experimental design. Creative Biolabs has developed a number of sensitive CAR detection reagents and related assays to verify CAR expression.

Fig.3 CAR expression test of CD99-CART cells measured by flow cytometry.¹

Anti-CD99 CAR-T Cytokine Release Test

Cytokine detection is used to characterize CART-related functions and confirm the target specificity and effectiveness of the CART cells. We provide a wide range of cytokine detection tests, such as ELISA, multiple cytokine profile arrays, and intracellular cytokine tests. Meanwhile, kinds of cytokines can be detected, including but not limited to IL-2, IFN-γ, IL-6, and IL-10.

Anti-CD99 CAR-T In Vitro Cytotoxicity Assay

In vitro tumor killing experiments can effectively illustrate the clearance ability of CART cells against target cells, providing relevant references for further in vivo experiments. Creative Biolabs has launched many sensitive cytotoxicity assays for global customers to choose from, including luciferase-based cytotoxicity tests, chromium (51 chromium) release assays, flow cytometry assays, and calcein release assays.

Fig.4 Cytotoxic assessment of the CD99 CAR-T cells against target cells from T-ALL patients' blasts at indicated E:T ratios.¹

Anti-CD99 CAR-T In Vivo Assays

Anti-CD99 CAR-T Cell Therapy Animal Models

According to different targets and project requirements, it is necessary to select appropriate animal models and corresponding experimental design schemes. With our years of experience in CART fields, we offer a wide range of relevant in vivo animal models to assist in CART development, such as CDX models, and PDX models.

Fig.5 Schematic of in vivo study design of anti-CD99 CAR-T in T-ALL CDX and PDX models.¹

Efficacy Test of Anti-CD99 CAR-T

Creative Biolabs provides comprehensive in vivo pharmacodynamic evaluation services to provide the scientific basis for your preclinical studies.

Fig.6 Efficacy assessment of CD99 CAR-T in Jurkat or MOLT-4 CDX models monitored by bioluminescent imaging.¹

Reference

1. Shi, Jiangzhou, et al. "CAR T cells targeting CD99 as an approach to eradicate T-cell acute lymphoblastic leukemia without normal blood cells toxicity." Journal of Hematology & Oncology 14.1 (2021): 1-5.