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Claudin18.2

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Product List Background Claudin18.2 CAR Design & Construction Anti-Claudin18.2 CAR-T In Vitro Assay Services Anti-Claudin18.2 CAR-T In Vivo Assay Services

Product List

Creative Biolabs provides extensive Claudin18.2 CAR-related products, you can choose the appropriate products that you desire for your research project.

Background

Claudin18.2 (CLDN18.2) is an isoform of claudin18 and a member of the tight junction protein family. Its expression is limited in normal tissues, while abnormal expression often occurs in the development of many malignant cancers, such as gastric cancer, colon cancer, breast cancer, liver cancer, and non-small-cell lung cancer. CLDN18.2 is involved in many important biological processes, such as the proliferation, differentiation, and migration of cancer cells. Featured with its specific expression pattern and crucial biological functions, CLDN18.2 has emerged as an attractive therapeutic target for many cancers.

Fig.1 Schematic diagram of the structure of Claudin18.2. (Chen, Jinxia, et al., 2023)

Fig.1 Schematic diagram of the structure of Claudin18.2.1

Associated Diseases

  • Gastric cancer
  • Liver cancer
  • Breast cancer
  • Head and neck cancer
  • Colon cancer
  • Others

Anti-Claudin18.2 CAR-T Services

Claudin18.2 CAR Design & Construction

With years of experience in CAR-T fields, Creative Biolabs specializes in providing various robust CAR constructions, such as classical CAR design, armored CAR constructions, etc.

Fig.2 Schematic diagram of Claudin18.2 CAR construction. (Shi, Hongtai, et al., 2023)

Fig.2 Schematic diagram of Claudin18.2 CAR construction.2

Anti-Claudin18.2 CAR-T In Vitro Assays

Anti-Claudin18.2 CAR Expression Assay

CAR expression detection is a very important step in CART development, so it is necessary to develop sensitive detection methods. Creative Biolabs has developed extensive CAR detection reagents (e.g., antigen-specific detection reagents, linker-specific detection antibody), as well as comprehensive detection assays to speed CART development.

Fig.3 CAR expression detection of anti-Claudin18.2 CAR-T cells by flow cytometry with representative plots (Left) and MFI percentage (right). (Shi, Hongtai, et al., 2023)

Fig.3 CAR expression detection of anti-Claudin18.2 CAR-T cells by flow cytometry with representative plots (Left) and MFI percentage (right).2

Anti-Claudin18.2 CAR-T Proliferation Detection

To detect the proliferation ability of CAR-T cells, we have launched various testing services to facilitate global customers' projects, including MTT assay, CCK-8 assay, flow cytometry analysis, and CFSE-based proliferation test, etc.

Fig.4 Expansion and viability analysis of anti-Claudin18.2 CAR-T cells. (Shi, Hongtai, et al., 2023)

Fig.4 Expansion and viability analysis of anti-Claudin18.2 CAR-T cells.2

Phenotypic Analysis of Anti-Claudin18.2 CAR-T

To characterize the functional performance of CART when co-cultured with target cells, Creative Biolabs offers comprehensive phenotypic analysis services to provide truly translational data. These assays include but are not limited to CART activation test, exhaustion test, T cell subgroup analysis, etc.

T cell Subpopulation Analysis

Fig.5 T cell subpopulation (TN, TCM, and TEM) analysis of in the CD8 (left) or CD4 (right) subsets of anti-Claudin18.2 CAR-T cells. (Shi, Hongtai, et al., 2023)

Fig.5 T cell subpopulation (TN, TCM, and TEM) analysis of in the CD8 (left) or CD4 (right) subsets of anti-Claudin18.2 CAR-T cells.2

Exhaustion Test of Anti-Claudin18.2 CAR-T

Fig.6 TIM-3 marker expression detection in anti-Claudin18.2 CAR-T cells on Day 7 after transduction. (Shi, Hongtai, et al., 2023)

Fig.6 TIM-3 marker expression detection in anti-Claudin18.2 CAR-T cells on Day 7 after transduction.2

Anti-Claudin18.2 CAR-T Cytokine Release Test

Cytokine release test is often used in assessing the target specificity and effectiveness of CAR-T cells against target cells. Creative Biolabs offers various techniques for cytokine tests, such as ELISA, multiplex bead arrays, and intracellular cytokine tests by flow cytometry.

Fig.7 Cytokine (IFN-γ, IL-2, and GM-CSF) analysis of anti-Claudin18.2 CAR-T cells incubated with different tumor cells at indicated E:T ratio. (Shi, Hongtai, et al., 2023)

Fig.7 Cytokine (IFN-γ, IL-2, and GM-CSF) analysis of anti-Claudin18.2 CAR-T cells incubated with different tumor cells at indicated E:T ratio.2

Anti-Claudin18.2 CAR-T In Vitro Cytotoxicity Assay

To assess the target specificity and tumor-killing capability of CART cells, Creative Biolabs offers various in vitro cytotoxicity assays for customers to choose from, including chromium (51 chromium) release assays, luciferase-based cytotoxicity tests, flow cytometry-based tests, etc.

In Vitro Cytotoxicity Analysis by Luciferase Assay System

Fig.8 In vitro cytotoxicity analysis of anti-Claudin18.2 CAR-T cells against different target cells at various E:T ratios by luciferase assay system. (Shi, Hongtai, et al., 2023)

Fig.8 In vitro cytotoxicity analysis of anti-Claudin18.2 CAR-T cells against different target cells at various E:T ratios by luciferase assay system.2

In Vitro Dynamic Killing Assay

Fig.9 In vitro dynamic killing assessment of anti-Claudin18.2 CAR-T cells against different target cells by RTCA assay. (Shi, Hongtai, et al., 2023)

Fig.9 In vitro dynamic killing assessment of anti-Claudin18.2 CAR-T cells against different target cells by RTCA assay.2

Anti-Claudin18.2 CAR-T In Vivo Assays

Selecting and appropriate animal model is crucial for the proof of concept of CAR-T cell function. Creative Biolabs offers a variety of animal models to help you test information about the efficacy, distribution, and proliferation characteristics of CAR T cells in vivo.

Efficacy Test in Panc02-Claudin18.2 CDX model

Fig.10 In vivo efficacy of anti-Claudin18.2 CAR-T in Panc02-Claudin18.2 CDX model. (Shi, Hongtai, et al., 2023)

Fig.10 In vivo efficacy of anti-Claudin18.2 CAR-T in Panc02-Claudin18.2 CDX model. (D) In vivo experimental design. (E) Tumor growth assessment of Panc02-Claudin18.2 CDX model treated with anti-Claudin18.2 CAR-T cells. (F) Tumor growth inhibition statistics of anti-Claudin18.2 CAR-T.2

Efficacy Test in B16F10-Claudin18.2 CDX model

Fig.11 In vivo efficacy of anti-Claudin18.2 CAR-T in B16F10-Claudin18.2 CDX model. (Shi, Hongtai, et al., 2023)

Fig.11 In vivo efficacy of anti-Claudin18.2 CAR-T in B16F10-Claudin18.2 CDX model. (G) In vivo experimental design. (H) Tumor growth assessment of Panc02-Claudin18.2 CDX model treated with anti-Claudin18.2 CAR-T cells. (I) Tumor growth inhibition statistics of anti-Claudin18.2 CAR-T.2

References

  1. Chen, Jinxia, et al. "Targeting CLDN18. 2 in cancers of the gastrointestinal tract: New drugs and new indications." Frontiers in Oncology 13 (2023): 1132319.
  2. Shi, Hongtai, et al. "IL-15 armoring enhances the antitumor efficacy of claudin 18.2-targeting CAR-T cells in syngeneic mouse tumor models." Frontiers in Immunology 14 (2023).
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CAT Product Name Target Species Antibody Clone Antibody Host Receptor Construction Vector Type Targeting Cell Type CAR Vector Type Inquiry & Datasheet
XS-0622-ZP3388 Anti-Claudin18.2 h(VHH1-VHH2-4-1BB-CD3ζ) Biepitopic CAR, pCDCAR1 VHH1-VHH2-4-1BB-CD3ζ Lentiviral vector T Cell   Add to Cart   Datasheet
XS-0922-ZP894 Anti-Claudin18.2 (CB-P14) h(CD28-CD3ζ) CAR, pCDCAR1 Mouse CB-P14 Rat scFv-CD28-CD3ζ Lentiviral vector T cell   Add to Cart   Datasheet
XS-0922-ZP1204 Anti-Claudin18.2 (CB-P14) h(4-1BB-CD3ζ) CAR, pCDCAR1 Mouse CB-P14 Rat scFv-4-1BB-CD3ζ Lentiviral vector T cell   Add to Cart   Datasheet
XS-1122-LX230 Anti-Claudin18.2 (X11X230) h(CD28-41BB-CD3ζ) CAR, pCDCAR1 Human X11X230 Mouse scFv-CD28-41BB-CD3ζ Lentiviral vector T cell   Add to Cart   Datasheet
XS-1122-LX621 Anti-Claudin18.2 (X11X223) h(CD28-OX40-CD3ζ) CAR, pCDCAR1 Human X11X223 Mouse scFv-CD28-OX40-CD3ζ Lentiviral vector T cell   Add to Cart   Datasheet
XS-1122-LX622 Anti-Claudin18.2 (X11X224) h(CD28-OX40-CD3ζ) CAR, pCDCAR1 Human X11X224 Mouse scFv-CD28-OX40-CD3ζ Lentiviral vector T cell   Add to Cart   Datasheet
XS-1122-LX623 Anti-Claudin18.2 (X11X225) h(CD28-OX40-CD3ζ) CAR, pCDCAR1 Human X11X225 Mouse scFv-CD28-OX40-CD3ζ Lentiviral vector T cell   Add to Cart   Datasheet
XS-1122-LX624 Anti-Claudin18.2 (X11X226) h(CD28-OX40-CD3ζ) CAR, pCDCAR1 Human X11X226 Mouse scFv-CD28-OX40-CD3ζ Lentiviral vector T cell   Add to Cart   Datasheet
XS-1122-LX625 Anti-Claudin18.2 (X11X227) h(CD28-OX40-CD3ζ) CAR, pCDCAR1 Human X11X227 Mouse scFv-CD28-OX40-CD3ζ Lentiviral vector T cell   Add to Cart   Datasheet
XS-1122-LX626 Anti-Claudin18.2 (X11X228) h(CD28-OX40-CD3ζ) CAR, pCDCAR1 Human X11X228 Mouse scFv-CD28-OX40-CD3ζ Lentiviral vector T cell   Add to Cart   Datasheet
XS-1122-LX627 Anti-Claudin18.2 (X11X229) h(CD28-OX40-CD3ζ) CAR, pCDCAR1 Human X11X229 Mouse scFv-CD28-OX40-CD3ζ Lentiviral vector T cell   Add to Cart   Datasheet
XS-1122-LX628 Anti-Claudin18.2 (X11X230) h(CD28-OX40-CD3ζ) CAR, pCDCAR1 Human X11X230 Mouse scFv-CD28-OX40-CD3ζ Lentiviral vector T cell   Add to Cart   Datasheet
XS-1122-LX1019 Anti-Claudin18.2 (X11X223) h(ICOS-4-1BB-CD3ζ) CAR, pCDCAR1 Human X11X223 Mouse scFv-ICOS-4-1BB-CD3ζ Lentiviral vector T cell   Add to Cart   Datasheet
XS-1122-LX1020 Anti-Claudin18.2 (X11X224) h(ICOS-4-1BB-CD3ζ) CAR, pCDCAR1 Human X11X224 Mouse scFv-ICOS-4-1BB-CD3ζ Lentiviral vector T cell   Add to Cart   Datasheet
XS-1122-LX1021 Anti-Claudin18.2 (X11X225) h(ICOS-4-1BB-CD3ζ) CAR, pCDCAR1 Human X11X225 Mouse scFv-ICOS-4-1BB-CD3ζ Lentiviral vector T cell   Add to Cart   Datasheet
XS-1122-LX1022 Anti-Claudin18.2 (X11X226) h(ICOS-4-1BB-CD3ζ) CAR, pCDCAR1 Human X11X226 Mouse scFv-ICOS-4-1BB-CD3ζ Lentiviral vector T cell   Add to Cart   Datasheet
XS-0123-ZP269 Anti-Claudin18.2 (CB-P15) h(scFv-CD3ε) TRuC, pCDTRC1 Human CB-P15 Mouse scFv-CD3ε Lentiviral vector T cell   Add to Cart   Datasheet
XS-0123-ZP523 Anti-Claudin18.2 (CB-P14) h(scFv-CD3ε) TRuC, pCDTRC1 Mouse CB-P14 Rat scFv-CD3ε Lentiviral vector T cell   Add to Cart   Datasheet
XS-0323-ZP269 Anti-Claudin18.2 (CB-P15 scFv-CD28TM-CD79β) CBCR(CAR-B), pCDCAR1 Human CB-P15 Mouse scFv-CD28TM-CD79β Lentiviral vector T cell CAR-B Vector   Add to Cart   Datasheet
XS-0323-ZP523 Anti-Claudin18.2 (CB-P14 scFv-CD28TM-CD79β) CBCR(CAR-B), pCDCAR1 Mouse CB-P14 Rat scFv-CD28TM-CD79β Lentiviral vector T cell CAR-B Vector   Add to Cart   Datasheet
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