• One-Stop CAR-T Therapy Development
  • Biomarker Identification & Selection
  • ScFv Generation
  • CAR Design & Construction
  • CAR-T Gene Packaging & Delivery
  • Replication-Competent Virus Testing
  • CAR Cell In Vitro Assay
  • CAR-T Preclinical In Vivo Assay
  • IND Development for CAR-T Cell Therapy
  • GMP Production for CAR-T Products
  • CAR Clinical Trial
  • One-Stop Canine CAR-T Therapy Development
  • One-Stop mRNA CAR-T Therapy Development
  • TRAC-CAR-T Cell Development
  • Modular CAR-T Cell Generation
  • One-Stop Silence™ CAR-T Cell Development
  • Vaccine Boosting CAR-T Cell Therapy
  • STAb-T Cell Therapy Development
  • CAR-T Therapy Development in the Non-Cancer Field
  • Anti-HIV CAR Cell Therapy Development
  • T Cell Activity Modulator Discovery
  • T Cell Programming
  • Virus Specific T Cell (VST) Development
  • Antibody-Coupled T Cell Receptor (ACTR) Development
  • Organoid-induced hPSC-original T Cell Differentiation
  • γδ T Cell Engineering
  • CAR-T CDMO
• One-Stop TCR-T Therapy Development
  • TCR Biomarker Identification & Selection
  • TCR Generation & Optimization
  • TCR Design & Construction
  • TCR Gene Packaging & Delivery
  • TCR In Vitro Assay
  • TCR-T Preclinical In Vivo Assay
  • TCR Clinical Application
  • TCR Analysis
  • TCR Tetramerization Construction
  • ScFv-TCR T Cell Therapy Development
  • AbTCR Development
  • Bispecific TCR Development
  • CER Development
• One-Stop CAR-MA Therapy Development
• One-Stop CAR-NK Therapy Development
  • One-Stop CAR-NKT Therapy Development
  • One-Stop UniCAR-NK Therapy Development
  • iPSC-derived CAR-NK Therapy Development
  • One-Stop TCR-NK Therapy Development
  • NK Cell Genetic Engineering
  • NK Cell Expansion
• One-Stop CAR-B Therapy Development
• One-Stop CAR-Monocyte Therapy Development
• One-Stop Cytokine Release Syndrome (CRS) Management
• TCR-Like Antibody Development
  • MHC/Peptide Complex Production
  • TCR-Like Antibody Discovery
  • TCR-Like Antibody Affinity Characterization
  • TCR-Like CAR Construction & Evaluation
• In Vivo Cell Engineering Development
  • In Vivo CAR-T Cell Engineering
  • In Vivo B Cell Engineering
  • In Vivo CAR-NK Cell Engineering
  • In Vivo CAR-M Cell Engineering
• One-Stop Allogeneic Cell Therapy Development
  • Allogeneic CAR-T Therapy Development
  • Allogeneic TCR-T Therapy Development
  • Allogeneic CAR-NK Therapy Development
  • Allogeneic CAR/TCR Cell Design & Engineering
  • Other Allogeneic Cell Therapy Development
• TCR/CAR/Antigen-Engineered Tregs Development
  • CAR-Treg Development
• Cancer Vaccine Development
  • Cancer Vaccine Discovery
  • Cancer Vaccine Engineering
  • Cancer Vaccine Analytical Characterization
  • Cancer Vaccine In Vivo Assessment
  • Cancer Vaccine GMP Manufacturing
  • Dendritic Cell Vaccine Development
• Immune Genotyping
  • HLA Typing
  • KIR Typing
  • HLA Testing
  • Dog DLA Genotyping
  • Guinea Pig GPLA Genotyping
  • Mouse H2 Genotyping
  • Rabbit RLA Genotyping
  • Rat RT1 Genotyping
  • Rhesus Macaque MHC Genotyping
• Other Services
  • Tumor-infiltrating Lymphocyte-based Antibody Discovery

TCR Gene Transfer Service

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Creative Biolabs offers various strategies to perform the transfer of TCR genes into primary T cells. Transferring an exogenous gene into T cells is a barrier impedes the development of TCR-modified T cells immunotherapy. However, with our up-to-date equipments, experienced project teams, we can develop the most appropriate strategy to achieve the effective gene transduction into T cells.

Gene transfer technologies offer the ability to genetically modify the cells. Generally, to choose the appropriate method of DNA transfer mainly depends on the target cells and the objectives of gene manipulation. There are two classes of approaches that can be used for DNA transfer. The first strategy utilizes natural methods to generate gene transfer, this class of methods includes conjugation, bacterial transformation, phage transduction, viral transduction, transposition and agrobacterium mediated transfer. DNA transfer can also be realized by artificial methods, which contains physical methods (macroinjection, microinjection, protoplast fusion and biolistics transformation), chemical methods (calcium phosphate, polyethene glycol, DEAD-Dextran and liposome) and electrical methods (electroporation and electrofusion). All these methods are powerful and ideal tools for scientific and clinical researches and have possible applications in gene therapy. In the development of TCR-modified T cells immunotherapy, the gene delivery is often done by treating cells with viral vectors such as retrovirus vectors and lentiviral vectors, or electroporation. The genome of virus has been manipulated to carry exogenous DNA. With engineering development, the immunogenicity of adenovirus vectors has been reduces which enhance their applications for long-term gene transfer in different tissues. Viral vectors used in scientific and clinical researches for gene therapy include oncoretroviruses (e.g. moloney murine leukaemia virus), lentiviruses, retroviruses, adenoviruses, adeno-associated viruses and herpes simplex virus-1. Using the method of viral vector-based gene transfer can increase the stability of integration of the viral genome into the host. Electroporation employs high-voltage electrical pulse to introduce DNA into cells.

Based on the type of transduced immune cells and the specific requirements of every client, scientists from Creative Biolabs will design the appropriate strategy to integrate modified TCR into the host cells.

The most popular strategies used for TCR gene transfer:

References:

1. K.H.KHAN. Gene Transfer Technologies and their Applications: Roles in Human Diseases. ASIAN J. EXP. BIOL. SCI. VOl 1 (1) 2010:208-218.
2. Clare E. Thomas. et al. Progress and problems with the use of viral vectors for gene therapy. Nature Reviews Genetics 4, 2003: 346-358.

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