Creative Biolabs is committed to providing our customers with high-level and high-quality complement research services with a professional and rigorous scientific spirit. Thanks to our outstanding technical team, our services cover many aspects of biology and molecular biology. Complement receptor 2 (CR2, CD21) is a receptor for specific complement component 3 cleavage fragments. According to research, CR2 is involved in the transport and retention of immune complexes to follicles, and acts as a co-receptor on the surface of B cells. Its ligand binding effectively reduces the activation threshold of B cells. Here we briefly describe a protocol for stimulating CR2 by flow cytometry and monitoring enhanced B-cell receptor Ca2+ mobilization protocol that allows coreceptor function analysis for CR2.
Protocol for Calcium Signaling Enhancement Quantification of CR2
Fig.1 Flow of monitoring enhanced B-cell receptor Ca2+ mobilization protocol. (Creative Biolabs)
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