This product is a 53.7 kDa recombinant human C3AR1 protein with a C-MYC/DDK tag expressed in the HEK293T Cells. It is available for WB, ELISA, PA.
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Expressed Host | HEK293T Cells |
Specie Reactivity | Human |
Target | C3aR |
Molecule Mass | 53.7 kDa |
Purity | >80% as determined by SDS-PAGE and Coomassie Blue staining |
Formulation | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol |
Applications | WB; ELISA; PA |
Product Form | Liquid |
Storage | Store at -20/-80°C. Aliquot to avoid repeated freezing and thawing. |
Introduction | C3a anaphylatoxin chemotactic receptor is the receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. |
Alternative Names | Complement C3a Receptor 1; Complement Component 3a Receptor 1; HNFAG09; AZ3B; C3AR; C3a Anaphylatoxin Chemotactic Receptor; Complement Component 3 Receptor 1; C3a-R; C3R1 |
Gene ID | 719 |
UniProt ID | Q16581 |
A valuable tool for our researchExcellentWe used it primarily for Western Blotting and were impressed with the protein's purity and consistency. It resolved well in our SDS-PAGE and showed clear, specific bands in our WB analysis. The tag facilitated the identification and purification process.
We observed high specificity and minimal background noiseExcellentThis protein has been instrumental in our ELISA assays. The MYC/DDK tag was particularly helpful for detection, allowing us to achieve reproducible and reliable results across multiple experiments.
The protein's performance in our assays was exceptionalExcellentThe MYC/DDK tag provided a robust signal, making it easier to track interactions with our target molecules. We encountered no issues with protein degradation, and the results were consistently high-quality.
Our research team has been using this C3AR1 protein for various assaysExcellentThe protein's purity was evident, and the MYC/DDK tag made detection straightforward. We experienced no issues with cross-reactivity, and the protein performed well even in complex cellular extracts. It's a dependable reagent for studying complement system components.
We were pleased with the protein in our experimental setupExcellentOur ELISA results were consistent, and the Western Blot analysis revealed clear bands with the expected molecular weight. It's been a reliable tool for our ongoing drug development studies.