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Electrophoretic Mobility Shift Assay (EMSA) Service

Principle of Electrophoretic Mobility Shift Assay (EMSA)

EMSA is a biochemical procedure for characterizing protein-nucleic acid interaction (PNI). Nucleic acid fragments are often labeled for visualization purposes. In a classical assay, labeled nucleic acid and test protein are incubated, and the resulting mixture is subjected to electrophoresis. Since the molecular mass of the complex formed increases, the mobility of the complex decreases during this process. Since nucleic acid contains some special markers, the distribution of the substance can be determined by various methods, thereby proving the PNI. With the advancement of experimental technology, the assay can perform qualitative and quantitative analyses of the target.

The schematic illustration of EMSA. Fig.1 The schematic illustration of EMSA. (Song, 2015)

Advantages of EMSA


What Can We Do?


EMSA is a popular in vitro method for detecting PNI. In addition to detecting transcription factors, this technique plays a crucial role in DNA-protein interactions, RNA-protein interactions and even DNA-RNA interactions. At Creative Biolabs, we offer specialized EMSA services to efficiently and accurately identify potential PNI.


During this assay, we add specific antibodies that recognize the protein of interest to the mixture to generate complexes with greater displacement. This method allows the identification of proteins present in the complex.


Running additional lanes with competing oligonucleotides is an effective strategy for determining the most favorable binding sequence of the binding protein. Furthermore, variants of MC-EMSA are powerful tools for measuring the specificity of binding as well as the measurement of association and dissociation kinetics.

Service Work Flow

The workflow of EMSA.Fig.2 The workflow of EMSA. (Creative Biolabs)

Why Choose Us?

Our advantages.Fig.3 Our advantages. (Creative Biolabs)

At Creative Biolabs, we conduct strict quality inspections on both the nucleotides and proteins to be tested to ensure the smooth progress of EMSA experiments. Our advanced signal detection instruments guarantee high sensitivity and high signal-to-noise ratio results. Please contact us in time to obtain professional EMSA service solutions. Our seasoned scientists are ready to start your project.

Reference

  1. Song, C.; et al. Choosing a suitable method for the identification of replication origins in microbial genomes. Frontiers in microbiology. 2015, 6: 1049.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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