Exosomes are membrane-derived particles surrounded by phospholipids released by cells. Labeling exosome with fluorescent dyes is an effective method for exosome visualization, which is beneficial to the research and application of exosomes. Creative Biolabs provides several cell labeling kits and exosome labeling kits, which can be used in most applications that require visualization of labeled exosomes for tracking studies. These products have the following advantages:
These products have the following advantages:
Specific
These products deliver a robust signal specific for exosome and very low levels of background signal.
Compatible
These products are suitable for delivering robust performance on exosomes isolated using all methods tested.
Easy-to-use
Protocols are quick and simple.
Sensitive
As little as 50 µg of exosomes
Double labeling
Matching method for co-localization:
EXA-0822-LX12 + EXA-0822-LX15
EXA-0822-LX13 + EXA-0822-LX14
A: Exosome labeling reagents can include fluorescent dyes, biotin, antibodies, nanoparticles and other types to achieve different labeling methods.
A: Exosome labeling can help researchers track the movement, localization and interactions of exosomes to better understand their role in cell communication and disease mechanisms.
A: The selection of exosome labeling reagents should be determined based on factors such as experimental purpose, cost, imaging equipment requirements, etc. Fluorescent dyes are often used for direct visualization, while biotin labels or other protein tags are suitable for subsequent detection or separation.
A: Factors that affect the labeling effect of exosomes include the concentration of labeling reagents, processing time, cell type, and the purity and functional status of exosomes.
A: If it is found that the labeling reagent has adverse effects on exosomes, you can try to optimize the experimental conditions, reduce the labeling concentration, select other labeling methods and other methods for improvement.
A: When storing and handling exosome labeling reagents, the manufacturer's instructions should be followed, and care should be taken to avoid prolonged exposure to light, high temperature, or freeze-thaw conditions that may affect the stability of the reagents.