Due to their native structural property and function in cell-cell communication, exosomes have the potential to be used as a carrier of gene and drug delivery for disease treatment. As a professional research organization and years of experience supplier in biotech markets, Creative Biolabs is devoted to offering high-level and cost-efficient customized exosome services for further understanding of exosome structure and biogenesis. Aided by our exosome engineering platforms and accurate results, researchers can rapidly and extensively refresh molecular mechanisms of exosome functions and expand insights into their practical potency.

Exosome Engineering

Exosomes are considered to be a new generation of drug delivery platforms due to their high delivery efficiency, low immunogenicity, good biocompatibility, and easy crossing of the blood-brain barrier. Due to the characteristics and biogenesis of exosomes that make them easy to modify, it is necessary to add specific surface molecules (such as molecules targeting specific tissues or anti-phagocytic proteins) on the basis of their own lipid bilayer structure for engineering. Both therapeutic molecules and molecules with specific functions can be modified in the lumen or surface of exosomes by different methods.

• Use various membrane penetration techniques (such as repeated freezing and thawing, ultrasound, electroporation, etc.) or modify the exosome production pathway in donor cells to load specific molecules in the exosome lumen.
• Use hydrophobic lipid-based complexes or modify the exosome generation pathway in donor cells (usually modifying exosome membrane surface proteins) to modify small therapeutic molecules or functional molecules (such as targeting peptides, antiphagocytic proteins, and fluorescent proteins) on the surface of exosomes.

Fig. 1 Common exosome engineering strategies.¹

Exosome Engineering Services at Creative Biolabs

Creative Biolabs provides a range of customized exosome engineering services. For example, performing fluorescent or biotin-labeled exosomes to drive detection of their uptake by cells, biodistribution, and tumor enrichment patterns. We can construct well-targeted designed exosomes for the active targeting of exosomal cargoes to a variety of organs, cells, and disorders along with developing proven strategies for loading desired cargoes into exosomes. We are also committed to providing services based on engineering modifications to explore the exosomal anti-phagocytic and enhance their circulation behaviors. Notably, we combine the advantages of multispecific antibodies with exosome vehicles, designing exosomes modified with multiple antibodies, which carry multiple sites of interaction with targets. This confers stronger targeted delivery effects to exosomes and produces more significant anti-tumor cytotoxicity by redirecting immune effector cells. In addition, we offer production services for exosome-associated AAV vectors to enhance gene delivery. Creative Biolabs has designed several specialty exosome engineering services, including but not limited to:

• Exosome Labeling
• Disease-Targeted Exosome Modification
• Exosome Cargo Loading
• Exosome-Associated AAV Vector Production
• Phagocytosis Escape-Reinforced Exosome Modification
• Multi-specific Antibody-modified Exosome Engineering

Features

• Tailored Cargo: Exosomes can be engineered to carry specific cargo, such as therapeutic molecules or diagnostic agents, thereby aiding research and development of their therapeutic or diagnostic potential.
• Targeted Delivery: Through surface modification, exosomes can be targeted to specific cell types or tissues, improving their efficiency and reducing off-target effects.
• Enhanced Stability: Engineering can confer increased stability to exosomes, prolonging their circulation time in the body and improving their overall efficacy.
• Scalable Production: Customized production processes enable the scalable manufacture of engineered exosomes, ensuring consistent quality and quantity for research applications.
• Safety Profiles: By modifying the exosome's surface or cargo, safety profiles can be optimized, reducing potential immunogenicity or off-target effects.

FAQs

Creative Biolabs is a technical service provider dedicated to the development and clinical transformation of exosomes. In terms of exosome modification, our professional technical team can provide the most comprehensive engineering exosome construction services, including pre-service consultation and all technical services from exosome isolation and identification to in vivo and in vitro functional research. Furthermore, a better understanding of exosome components and their activity patterns is necessary before utilizing exosome engineering to develop therapeutic drugs. Therefore, we can also provide customers with systematic exosome analysis technology to comprehensively understand the content of protein, lipid, and RNA molecules in exosomes through high-throughput multi-omics solutions to design effective exosome-based drug carriers. If you want to build innovative engineered exosomes, please contact us.

Reference

1. Xu, Meng, et al. "Engineered exosomes: Desirable target-tracking characteristics for cerebrovascular and neurodegenerative disease therapies." Theranostics 11.18 (2021): 8926. Under Open Access license CC BY 4.0, without modification.

• Exosome Isolation
• Exosome Purification
• Exosome Characterization
• Exosome Quantification
• Exosome Profiling Services
• Exosomal Proteomic Detection Services
• Exosome Manufacturing Services
• Exosome Antibody Development Services
• Exosome Display
• Extracellular Vesicle Related Services
• Single-Cell & Exosome Combined Research
• Tissue Exosome Related Services
• LipidSync Exosomes Development Services
• Exosome Lipidomics & Metabolomics Services
• Exosome - NGS Services
• In Vitro Functional Services of Exosomes
• In Vivo Functional Services of Exosomes