Exosome Characterization
As crucial mediators in cell-to-cell communication, exosomes play an essential role in maintaining physiological homeostasis and disease progression, revealing their significant potential in the fields of diagnosis and therapy. Consequently, researchers are dedicated to extracting exosomes from body fluids or cell culture media and exploring their application prospects. To delve deeper into the unique characteristics of exosomes and pave the way for subsequent therapeutic and diagnostic research, Creative Biolabs has introduced a range of advanced exosome characterization technologies, including nanoparticle tracking analysis, TEM, FACS, western blotting, MS, and TRPS.
Exosome Characterization Services in Creative Biolabs
Nanoparticle tracking analysis is a highly sensitive method for visualization and analysis of exosomes. This method directly visualizes the light which is scattered from an exosome in liquid suspension. A finely focused laser beam passes through an optical interface such that it refracts through a thin layer of sample in liquid suspension. Light is scattered by the exosome in the path of the laser beam and is collected using a light microscope, and then imaged. Plots of scattered light spots and their speed of motion provide the data that facilitate the determination of total particle count and size distribution.
TEM is an advanced technique that uses electron beams to penetrate samples to obtain high-resolution images, making it a cornerstone in the identification of exosomes. Through TEM, researchers can directly and clearly observe the morphology and size of exosomes, thus obtaining intuitive evidence about exosomes. TEM not only helps researchers confirm the presence of exosomes in samples but also allows for the assessment of the purity and integrity of exosomes, which is crucial for further functional studies of exosomes.
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Exosomal Markers Detection-based Exosome Characterization
Exosomes are characterized by their unique membrane proteins and internal contents. The detection of specific exosomal markers, such as membrane proteins CD63, CD9, CD81, and internal protein TSG101, enables the confirmation of exosomes' presence in samples and the assessment of the purity of exosome samples. Moreover, exosomes secreted by different cell types may express distinct marker proteins. By identifying these specific markers, researchers can differentiate and identify exosomes originating from diverse cellular sources.
1. Fluorescence-activated cell sorting (FACS)
FACS is commonly used for cell categorization and analysis, but it can also be employed to detect surface markers on exosomes at the individual exosome level. By utilizing fluorescently labeled antibodies that specifically bind to certain markers on the exosome surface, FACS can effectively identify and analyze specific proteins present on the surface of exosomes.
2. Western blotting
This method is a widely used analytical technique for detecting specific proteins in exosome, as well as semi-quantitative estimation of protein derived from the size and color intensity of a protein band on the blot membrane. Thus, western blotting has been utilized to identify the presence and expression level of a specific single protein in exosomes.
3. Enzyme-linked immunosorbent assay (ELISA)
ELISA is a commonly used analytical biochemistry assay. It uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a specific single protein on the surface of exosomes using a single or a pair of antibodies.
MS is an analytical technique that ionizes chemical species and sorts the ions based on their mass-to-charge ratio. This method can be used to identify protein cargo in exosomes and determine whether the sample may contain protein aggregates or other contaminants.
TRPS is a technique that allows high-throughput single particle measurements as colloids and/or biomolecular analytes driven through a size-tunable nanopore, one at a time. This method can be used to measure the size of exosome.
Features of Our Services
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Advanced technology platforms
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Accurate experimental data
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Highly experienced professionals' team
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Whole-process technical trace service
Based on the advanced technological platform, we can assist you to accurately analyze the characteristics of exosome derived from various sources. When you choose the exosome characterization of Creative Biolabs, you attain more than high-quality data delivery, you get in-depth scientific and technical support on everything from project planning to data interpretation from scientists with years of experience. If you have any demands in exosome characterization, please don't hesitate to contact us for more information.
For Research Use Only. Cannot be used by patients.
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