Exosome Isolation Services

Overview Services Features FAQs

Overview

With an unwavering commitment to excellence, Creative Biolabs offers you the opportunity to access exosomes from a wide range of biological samples, empowering your research endeavors with versatility and precision. Whether you're pursuing biomarker discovery, functional studies, or comparative analysis, our comprehensive services ensure that you have the right exosome samples to fuel your research goals.

Services

Table 1 Summary of various types of exosomes that can be isolated.

Specific Service Items	Description	Application Prospects
Animal Cell-derived Exosome Isolation	Exosomes derived from animal cells are tiny cell secretions. Almost all animals can release exosomes, such as stem cells, B cells, dendritic cells and mast cells, and tumor cells. These animal cell-derived exosomes play important roles in intercellular communication, immune response, developmental regulation, and disease progression.	Because the biomolecules carried by animal cell-derived exosomes can transmit information between cells, researchers are increasingly studying animal cell exosomes, hoping to use them to develop new treatments, diagnostic tools, and biomarkers.
Body Fluid-derived Exosome Isolation	Exosomes sourced from bodily fluids typically refer to vesicles present in the liquid environments within organisms, such as plasma, serum, urine, cerebrospinal fluid, and interstitial fluid.	Research into exosomes derived from these bodily fluid sources is continuously advancing, as scientists explore their roles in physiological and pathological processes, as well as how to leverage them for the development of novel diagnostic, therapeutic, and monitoring approaches.
Plant-derived Exosome Isolation	Plant-derived exosomes are a type of cell-secreted substance similar to animal cell-derived exosomes.	It has been discovered that exosomes from various plant sources hold potential applications in disease treatment, health care, and skin improvement. Scientists have begun to gradually unveil their functionalities and potential uses.
Microorganism-derived Exosome Isolation	Microorganisms can also release exosomes through fusion or secretion pathways. Microorganism-derived exosomes play a significant role in microbial communication, pathogenicity, and symbiotic relationships.	Researchers are actively exploring the functionalities of microorganism-derived exosomes, including their roles in disease development and host immune responses. These studies offer potential opportunities for developing new medical and biotechnological applications.

Table 2 Summary of various methods for exosome isolation.

Methods	Principle	Schematic Diagram	Advantages	Disadvantages
Ultracentrifugation	Exosomes are separated based on their density using high-speed centrifugation.	Fig.1 Schematic representation of differential ultracentrifugation-based exosome isolation.^1,2	Established and widely used method can purify exosomes from different biological fluids.	Labor-intensive, time-consuming, may co-precipitate non-exosome materials, potential damage to exosomes due to high forces.
Density Gradient Centrifugation	Exosomes are separated based on their buoyant density by gradient centrifugation.	Fig.2 Schematic representative of gradient density ultracentrifugation-based exosome isolation.^1,2	Good purity.	Complex, requires careful gradient preparation, longer procedure.
Ultrafiltration	Exosomes are filtered through a membrane with a specific pore size to separate them from larger particles.	Fig.3 Schematic of ultrafiltration-based exosome isolation.^1,2	Simple and relatively quick, suitable for large-volume samples.	This may result in co-isolation of proteins and contaminants.
Size-Exclusion Chromatography (SEC)	Exosomes are separated based on size by passing through a porous column.	Fig.4 Schematic of size-exclusion chromatography-based exosome isolation.^1,2	The gentle method that preserves exosome integrity.	Possible co-elute with proteins, which requires specialized equipment.
Precipitation	Polymers are added to induce exosome aggregation and precipitation.	Fig.5 Schematic of aqueous two-phase system-based exosome isolation.^1,2	Easy and rapid process, good yield from various biological fluids.	Contamination with polymers, potential disruption of exosome integrity.
Affinity-based Capture	Antibodies are used to capture exosomes based on specific surface markers.	Fig.6 Schematic of immunoaffinity-based exosome isolation.^1,2	High specificity, enables targeted isolation of exosome subpopulations.	Limited by the availability of well-characterized antibodies, potential loss of non-targeted exosomes.
Microfluidics-based Isolation	Microfluidic devices sort and isolate exosomes based on size, surface markers, or acoustic properties.	Fig.7 Integrated microfluidic technique allows combined exosome isolation and analysis.^1,2	Precise control, potential for automation, suited for rare exosome subpopulations.	Complex setup may require technical expertise.

Features

FAQs

Q: What types of exosome isolation services does your company offer?

A: We provide a variety of exosome isolation services, including extraction from blood, urine, culture mediums, and other biological samples. We utilize multiple techniques such as ultracentrifugation, size exclusion chromatography, and immunoaffinity purification to ensure the most optimized separation scheme for your specific research needs.

Q: What are the benefits of using your exosome isolation services?

A: Our services are highly specialized and customizable, able to be tailored to your specific requirements. We employ advanced isolation techniques to ensure high purity and yield, while minimizing sample contamination. Additionally, we provide detailed separation reports and technical support to aid your understanding and use of the isolated exosomes.

Q: What applications can the isolated exosomes be used for?

A: The isolated exosomes can be used for a variety of biomedical research applications, including but not limited to studies on cancer, neurodegenerative diseases, cardiovascular diseases, as well as potential applications in regenerative medicine and drug delivery systems. Furthermore, exosomes can be used for biomarker identification, offering potential for early disease diagnosis and treatment monitoring.

Q: How do you ensure that the exosomes are of high quality?

A: We adhere to strict quality control processes and use advanced analytical techniques such as nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) to confirm the size, concentration, and purity of the exosomes.

References

Yang, D.; et al. Progress, opportunity, and perspective on exosome isolation-efforts for efficient exosome-based theranostics. Theranostics. 2020, 10(8):3684-3707.
Distributed under Open Access license CC BY 4.0, without modification.