Exosome Quantification

Overview Technologies Features FAQs

The small size and physicochemical heterogeneity of exosomes make their physical characterization and quantification an extremely challenging task. Creative Biolabs has successfully launched an innovative exosome quantification platform with multiple high-end technologies to detect and quantify exosomes in complex biological samples.

Exosome Quantification

Exosomes are small vesicular structures that represent a novel source of biomarkers for the state of the cells from which they are derived. Typically, quantification is very important to understand basic biological relationships between exosomes and their parent cells, as well as the underlying interpretation of exosome signals. As interest in exosomes expended, the number of methods for quantifying exosomes has increased. In previous studies, three groups have illustrated the importance of quantifying exosomes, examining the stoichiometry of miRNAs per exosome. For example, Akers et al. isolated extracellular vesicles, primarily exosomes, from cerebrospinal fluid (CSF) samples and then measured the number of exosomes via nanosight nanoparticle tracking analysis and the total content of specific miRNAs by qRT-PCR. Creative Biolabs offers a variety of techniques for exosome quantification to satisfy our customer’s needs.

High-end Technologies to Quantify Exosome

Quantification is essential to understand basic biological relationships between exosomes and their parent cells and hence the underlying interpretation of exosome signals. Creative Biolabs offers robust technologies to fulfill the demands.

Nanoparticle Tracking Analysis
Nanoparticle Tracking Analysis provides a combined analysis of Brownian motion via light scattering to count and size nanoparticles in a liquid suspension. Particles in suspension passed through a flow chamber and are illuminated using a laser source. The light scatter produced from this is recorded using a video camera.
Tunable Resistive Pulse Sensing (TRPS)
Tunable resistive pulse sensing (TRPS) detects the passage of individual particles through a pore in a membrane, and operates similarly to a Coulter counter. TRPS detection provides both concentration and size information.
ELISA
Exosomes are captured at the bottom of a microwell and the amount of biomarker detected with antibodies generating a fluorescent or colorimetric signal. With the aim to provide a quantitative estimate of the amount of exosomal vesicle (EV) material carrying a specific marker from such assays, attempts have been made to relate specific protein content to total particle content using calibration curves established with characterized exosomal samples.

Fig.1 Chip design and principle for exosome capture and detection.^1,2
Vesicle Flow Cytometry
Flow cytometry detects particles suspended in a fluid by their interaction with a laser beam as they flow through a detection cell. Flow cytometry can take advantage of fluorescent-based immunostaining to provide an individual count of vesicles in solution characterized by the presence of specific markers.
Surface Plasmon Resonance
Surface-based sensors are used to determine the vesicle concentration in solution by measuring the rate of arrival of the vesicles to a sensor surface under mass transport limited conditions. Under such conditions, the initial binding rate is directly proportional to the concentration and the diffusion coefficient of the vesicles.

Features of Our Services

Highly efficient
One-stop pipeline
Skillful scientific team
Best after-sale service

With the help of our well-established technologies and experienced scientists, Creative Biolabs is capable of detecting and quantifying exosome in complex biological samples. We provide very flexible options for each specific case. We fully understand the advantages and limitation of each technology, and will help you choose the most appropriate technology to meet your specific needs. We are happy to make it accessible to all kinds of research and industrial customers. Please don’t hesitate to contact us for more information.

References

Fang, S.; et al. Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification. PLoS One. 2017, 12(4): e0175050.
under Open Access license CC BY 4.0, without modification.

FAQs

Why is quantifying exosomes important in research and drug development?

Quantifying exosomes provides critical information about their concentration, facilitating downstream experiments such as biomarker discovery, functional studies, and therapeutic development. Accurate quantification ensures reproducibility and reliability of experimental results, advancing research in understanding exosome biology and their applications in drug development.

How do exosome quantification services ensure accurate and reliable results?

Exosome quantification services employ validated protocols and state-of-the-art instrumentation to ensure accuracy and reliability. By standardizing procedures and calibrating instruments, we minimize variability and ensure reproducibility across experiments. Quality control measures and validation assays further verify the accuracy of quantification data, providing researchers with trustworthy results.

Can exosome quantification services be tailored to different sample types and concentrations?

Yes, our exosome quantification services are adaptable to various sample types and concentrations. Whether analyzing cell culture supernatants, biofluids, or tissue lysates, we optimize protocols and instrument settings to accommodate different sample characteristics and ensure accurate quantification across a wide range of exosome concentrations.

How does exosome quantification support the development of exosome-based therapeutics?

Exosome quantification is crucial for determining the optimal dosage and assessing the efficacy of exosome-based therapeutics. By accurately quantifying exosome concentration, researchers can establish dose-response relationships, monitor exosome biodistribution, and evaluate therapeutic outcomes, guiding the development of safe and effective exosome-based treatments in drug development.

Can exosome quantification services assist in comparing exosome yields from different isolation methods?

Yes, exosome quantification services enable researchers to compare exosome yields obtained from different isolation methods. By quantifying exosome concentration, researchers can evaluate the efficiency and purity of isolation techniques, identifying the most suitable method for their specific research needs and optimizing exosome isolation protocols for downstream applications in drug development.

Plant Exosome Isolation