Exosome Quantification Service
Overview Services Features FAQs
Overview
The small size and physicochemical diversity of exosomes make their physical characterization and quantification exceptionally challenging. Creative Biolabs has introduced an innovative exosome quantification platform that utilizes multiple advanced technologies to detect and quantify exosomes in complex biological samples.
Exosome Quantification
Exosomes are small vesicular structures that serve as a novel source of biomarkers reflecting the state of their parent cells. Accurate quantification is crucial for understanding the fundamental biological relationships between exosomes and their originating cells, as well as for interpreting exosome signals. As interest in exosomes has grown, so has the number of methods for their quantification. Previous studies have highlighted the importance of exosome quantification, examining the stoichiometry of miRNAs per exosome.
Services
Quantification is essential for understanding the basic biological relationships between exosomes and their parent cells, and thus for interpreting exosome signals. Creative Biolabs offers robust technologies to fulfill these demands.
NTA combines analysis of Brownian motion with light scattering to count and size nanoparticles in a liquid suspension. Particles pass through a flow chamber and are illuminated by a laser source, with the resulting light scatter recorded using a video camera.
TRPS detects individual particles passing through a membrane pore, functioning similarly to a Coulter counter. This method provides both concentration and size information.
In ELISA, exosomes are captured at the bottom of a microwell, and biomarkers are detected using antibodies that generate a fluorescent or colorimetric signal. This assay aims to provide a quantitative estimate of exosome carrying specific markers, relating specific protein content to total particle content using calibration curves from characterized exosome samples.
Nano Flow Cytometry detects particles in fluid based on their interaction with a laser beam as they flow through a detection cell. This technique can use fluorescent-based immunostaining to provide individual counts of vesicles characterized by specific markers.
Features
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Highly efficient
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One-stop pipeline
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Skillful scientific team
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Best after-sale service
With the help of our well-established technologies and experienced scientists, Creative Biolabs is capable of detecting and quantifying exosome in complex biological samples. We provide very flexible options for each specific case. We fully understand the advantages and limitation of each technology, and will help you choose the most appropriate technology to meet your specific needs. We are happy to make it accessible to all kinds of research and industrial customers. Please don't hesitate to contact us for more information.
FAQs
Q: Which technology is most suitable for exosome quantification?
A: For exosome quantification, we recommend nanoparticle tracking analysis (NTA) and tunable resistive pulse sensing (TRPS) as our top choices, both excelling in accuracy and reliability. For measuring the quantity of exosome subpopulations, enzyme-linked immunosorbent assay (ELISA) and nanoparticle flow cytometry are also excellent options. If you have doubts about technology selection, please contact our professional team. We will provide you with the most suitable technology recommendation based on your sample type, quantity, and accuracy requirements.
Q: What is the cost of exosome quantification services?
A: The cost of exosome quantification services varies depending on the number of samples, the chosen techniques, and other experimental conditions. You can contact our team, provide detailed research requirements, and we will provide you with corresponding quotations and cost estimates.
For Research Use Only. Cannot be used by patients.
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