Exosome antibodies are antibodies developed against exosomal surface markers. They have been widely used as exosome research tools and for clinical applications such as disease prognosis and diagnosis. To help promote the exosomal studies and their clinical use, Creative Biolabs provides high-quality antibody development services using different technologies, including phage display antibody screening and B cell sorting, which can help the discovery of antibodies that recognize exosome-associated antigens with sufficient sensitivity and specificity.
Exosomes, 30-150 nm in diameter, are endocytic membrane-derived vesicles that harbor a variety of proteins, nucleic acids and lipids, and are present in almost all bodily fluids. Many of the exosomal proteins are inserted in the membrane surface and some proteins that are most frequently identified are accepted as specific exosome markers, such as tetraspanins (e.g., CD9, CD63, and CD81 ), membrane transporters and fusion proteins (e.g., GTPases, annexins, and flotillin), and MVB biogenesis proteins (e.g., Alix and TSG101). Thus, antibodies against these markers can be developed for exosome studies. Moreover, some markers are disease-specific, making them potential biomarkers for disease diagnosis.
Fig.1 Molecular composition of exosomes. (Bellingham, 2012)
Premade Phage Display Antibody Library Screening
We offer world-class premade phage display antibody libraries for the screening of antibodies with high specificity and affinity. We provide a comprehensive portfolio of premade antibody libraries in the form of scFv, Fab, and VHH, originating from human, mouse, rabbit, chicken, camel, and llama. Premade phage antibody library requires no immunization.
Immune Phage Display Antibody Library Screening
Constructing and screening immune antibody library via phage display technology has become a major choice for monoclonal antibody production. Unlike premade phage antibody libraries, animals are firstly immunized with exosome-associated antigens to produce antibody-secreting B cells and experience the in vivo affinity maturation process. This way, monoclonal antibodies with high specificity and affinity can be obtained more easily.
B Cell Sorting for Exosome Antibody Development
B cell sorting technology is a useful approach to develop antibodies for diagnostics, pharmacokinetic studies, and clinical development. This technology is based on first isolation of single B cells from a large population of primary B cells, followed by the direct amplification of VH and VL region encoding genes and their subsequent expression in cell culture systems.
Creative Biolabs has the years of experience in exosome research and provided a full range of exosome-related services for many years. Our service program covers every step of exosome research including exosome isolation, characterization, quantification, engineering and so on. Please feel free to contact us for more details. If you can’t find your interested services on exosome service list, please also contact us to discuss your project and create a custom quote.
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Antibodies are essential tools for studying exosomes, as they enable specific detection and characterization of exosome surface markers or cargo molecules. This information is crucial for understanding exosome biology, function, and potential therapeutic applications.
Exosome antibody development involves generating antibodies against specific exosome surface markers or cargo molecules through immunization, hybridoma technology, or phage display. These antibodies are then validated for their specificity and affinity to ensure accurate detection and analysis of exosomes.
Exosome markers targeted for antibody development include tetraspanins (e.g., CD9, CD63, CD81), heat shock proteins (e.g., HSP70, HSP90), and cell-specific markers (e.g., EpCAM for epithelial-derived exosomes). These markers facilitate exosome isolation, characterization, and functional studies.
Exosome antibodies undergo rigorous validation assays, including Western blotting, flow cytometry, immunofluorescence, and ELISA, to confirm their specificity and functionality. These assays ensure accurate detection and quantification of exosomes in various biological samples.
Yes, our antibody development services offer customization options to generate antibodies targeting specific exosome markers or cargo molecules based on your research requirements. We work closely with clients to design and validate custom antibodies for precise exosome analysis.