Exosome Display

Overview Services Features FAQs

Exosomes are natural carriers, and this property has been used in the genetically engineered molecular composition of exosomes with foreign proteins and ligands. When displayed on the surface of exosomes, these molecules show increased solubility/functionality and can be used for a wide range of applications, including drug discovery, ligand screening, and targeted drug delivery. Creative Biolabs utilizes this technology, termed exosome display, for different research purposes and to promote clinical applications.

Introduction of Exosome Display

The protein contents of exosomes can be manipulated and tailored to endow the exosomes with new desirable properties. Specifically, molecules can be genetically fused to the exosomal transmembrane proteins, expressed on the surface, and endowed with various physiological properties, such as conferring targeting capability, exhibiting increased expression levels, offering enhanced solubility and extended half-life, and triggering antigen immunogenicity. These advantageous properties of recombinant exosomes can be applied to different applications, such as antibody generation, targeted drug delivery for gene therapy, as well as ligand screening of G protein-coupled receptors (GPCRs).

Fig. 1 Illustration of exosome structure and associated proteins and miRNAs.¹

Exosome Display Services

Exosome Display-based Antibody Generation & Isolation (ExoMAb)

Antibodies targeting a variety of targets, including GPCRs, can be produced by exosome display technology. These targets include integral membrane proteins, soluble proteins, and the extracellular domains of receptors. On one hand, the former two can be addressed to the surface of exosomes by fusing to targeting molecules, such as the tetraspanins. Antigens such as tumor biomarkers (e.g., cytokines) can be displayed for induction of antibody response. On the other hand, GPCRs can be displayed for drug discovery. Additionally, new avenues for developing innovative treatments and creating innovative diagnostic tools are made possible by this technology.

Exosome Display-based Targeted Delivery

Exosome targeting is made possible by ligand fragments and homing peptides addressed on the exosomal surface. This capacity has been utilized to deliver chemotherapeutic medications and RNA therapies (such as siRNAs and miRNA) in a targeted manner. The targeting moieties can be chosen based on the interested tissues, and the exosomal membrane protein selected as fusion partners also varies.

Exosome Display-based Protein Array Screen

Recombinant exosomes carrying potential therapeutic targets could also be utilized to screen cognate ligands or small compounds, as Exosome Display offers a simple platform to present membrane proteins in their original shape and surroundings. For instance, recombinant exosomes expressing GPCR may potentially be used in protein array screens to identify new drugs.

Features

Excellent quality, affordable cost, and trustworthy and reliable service
World-class technology platform
Professional technical team specialized in exosome display
Best after-sale service

Creative Biolabs is a world-leading company that provides a full range of exosome-related services ranging from exosome isolation to engineering. Please contact us to discuss.

FAQs

Q: What distinguishes exosome display from other display technologies?

A: Exosome display involves engineering exosomes to present foreign proteins or peptides on their surface for various applications, including drug targeting and vaccine development. Unlike other display systems, exosome display offers natural vesicle-based delivery and potential immunogenicity advantages.

Q: How are exosomes engineered for display purposes?

A: Exosome display utilizes genetic engineering techniques to fuse target proteins or peptides with exosome surface proteins (e.g., tetraspanins). These engineered proteins are then expressed in exosome-producing cells, leading to their incorporation into exosome membranes for surface presentation.

Q: What types of molecules can be displayed on exosomes?

A: Exosome display can present a wide range of molecules, including therapeutic proteins, antigens, peptides, and targeting ligands. This versatility enables applications such as targeted drug delivery, immunotherapy, and vaccine development using engineered exosomes as carriers or immunogens.

Q: How are exosomes displaying specific molecules characterized and validated?

A: Characterization of exosomes displaying specific molecules involves assessing their surface expression, stability, cargo loading efficiency, and functionality. Validation methods include flow cytometry, immunoblotting, electron microscopy, and functional assays to ensure accurate display and biological activity.

Q: Can exosome display services be customized for specific research needs?

A: Yes, our exosome display services offer customization options to tailor exosome engineering strategies and target molecule selection based on specific research goals. Whether it's optimizing display efficiency, selecting appropriate targeting ligands, or designing immunogenic payloads, we provide tailored solutions.

Reference

Chen, Qiao Yi, et al. "Exosomal proteins and miRNAs as mediators of amyotrophic lateral sclerosis." Frontiers in Cell and Developmental Biology 9 (2021): 718803. Under Open Access license CC BY 4.0. The image was modified by revising the title.

Plant Exosome Isolation