AAV Biodistribution and Shedding Analysis Service
The design of nonclinical studies to test the safety of biotechnology-derived pharmaceutical products is of great vitality. Recently, adeno-associated viral (AAV) vectors are widely used for in vivo gene therapy applications due to their excellent safety and efficacy profiles. As a pioneer company in the field of gene therapy, Creative Biolabs is capable of offering AAV biodistribution and shedding analysis service in the most high-quality and cost-effective way.
Importance of AAV Biodistribution and Shedding Analysis
Biodistribution refers to the spread of the vector DNA after administration, and its localization and persistence in tissues, body fluids or organs. Shedding may be observed as a consequence of biodistribution. The former is the dissemination of the vector DNA via body secretions including excreta, and the latter more specifically concerns the presence of vector DNA in the gonads that could potentially be transferred to progeny. It has been proved that possible expression of the transgene in non-target tissue is a drawback for gene therapy. Particularly, when screening candidate vectors, accurate knowledge about which tissues will be distributed is essential. Therefore, assessment of biodistribution and shedding should be carried out on each particular gene therapy product during its nonclinical and clinical evaluation stages.
Figure 1. Biodistribution of AAV vector following mesenteric and peripheral vein administration of scAAV2/8-LP1-hFIXco. (Nathwani, 2007)
Services
Working in the field of gene therapy for years, Creative Biolabs has developed a full package of techniques for AAV biodistribution and shedding analysis. With professional scientists, we can assess specific AAV vector distribution at different levels, including but not limited to organism level, tissue level, as well as the cellular level. The AAV biodistribution and shedding analysis services we offer include in vivo imaging (bioluminescence generated by luciferase expression), quantification of viral nucleic acids (typically by quantitative polymerase chain reaction), and fluorescence detection (expression of a green fluorescent protein or other fluorescent protein). Especially, the biodistribution and shedding analysis service we offer could be performed both on a "research" mode and within the frame of the regulatory animal pharmacology and toxicology studies.
Features of Our Services
- Multiple animal species available
- Experienced scientists for accurate data analysis
- Standard and customized experiment plan
- Best after-sale service with a relatively low price
Creative Biolabs is dedicated to offering AAV biodistribution and shedding analysis services for global customers. Following the current FDA guidelines, our experienced scientists will design the most appropriate experiment plan to meet every client's specific requirements. For more detailed information, please feel free to contact us or directly send us a quote.
Reference
- Nathwani, A.C.; et al. (2007). Safe and efficient transduction of the liver after peripheral vein infusion of self-complementary AAV vector results in stable therapeutic expression of human FIX in nonhuman primates. Blood. 109(4), pp.1414-1421.
