GTOnco™ Immune Cell-mediated Tumor Lysis Assay
Leveraging our extensive expertise in the immunotherapy field, Creative Biolabs provides analysis and interpretation of the effects of gene therapy-based I-O candidates for T cell-mediated tumor cells lysis. At GTOnco™, we are able to conduct powerful in vitro immune cell-mediated tumor lysis assay to validate the antitumor activity of lymphocytes. The co-cultured ratio of targeted tumor cells and lymphocytes will be optimized in the assay process. In addition, the targeted tumor cells are also labeled and monitored at a real-time level to measure the tumor cell lysis. We are pleased to share our previous experiences and work closely with our clients to tailor the assays to best address their requirements.
Figure 1. T Cell-mediated Tumor Lysis Assay.
Currently, there are two commonly used approaches to validate the immune cell-mediated tumor cell lysis, namely the radiolabeling method and in vitro live-cell imaging assay. In briefly, the targeted tumor cells are labeled with specific radiolabel or fluorescent cytoplasmic reagent, and then recognized by lymphocytes. Through real-time monitoring the change of radiolabel or fluorescence intensity in targeted tumor cells, the immune cell-mediated tumor cell lysis can be measured. Of course, we also provide the ratio optimization of the co-culture lymphocytes and tumor cells to quantify cytolytic activities of lymphocytes against target cells. GTOnco™ services can be also designed to meet our clients' special needs to support their gene therapy-based I-O products development.
As a leading life science service provider, Creative Biolabs has successfully established advanced in vitro validation platform for our clients all over the world. our scientists will professionally perform the immune cell-mediated tumor lysis assay to verify the anti-tumor activity of I-O products. Please feel free to contact us and we will get back to you as soon as possible.
Reference
- Cerignoli, F.; et al. (2018). In vitro immunotherapy potency assays using real-time cell analysis. PLOS ONE. 13(3), p.e0193498.
