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In Vitro Protein Evolution by Ribosome Display

Because of several advantages over other cell-based display systems, ribosome display has been confirmed as a powerful method for directed evolution of protein stability and affinity. Based on this technology, Creative Biolabs offers ribosome display library construction and screening as well as protein evolution services for our worldwide customers.

Protein Evolution by Ribosome Display

Ribosome display is a cell-free system for in vitro selection and evolution of protein and antibody from large libraries. It uses the principle of coupling individual nascent proteins (phenotypes) to their corresponding mRNA (genotypes), through the formation of stable protein-ribosome-mRNA (PRM) complexes. Compared to other cell-based systems, such as phage display, ribosome display has two important advantages for identifying and optimizing proteins. Firstly, the diversity of ribosome display libraries (1013~1014) is not limited by the transformation efficiency of bacterial cells, which allows the construction of a larger library. Secondly, random mutations can be introduced easily into the selected DNA pool in subsequent cycles, which allows facile directed evolution of binding proteins over several generations. Now a number of studies have been confirmed the potential of ribosome display for directed in vitro protein evolution. Hanes et al. (2000) improved the affinities of a series of scFv antibodies by 40 times by ribosome display technology. Jermutus et al. (2001) demonstrated that the affinity and stability of scFvs can be rapidly and efficiently evolved by ribosome display.

Schematic representation of a ribosome display selection round. Fig.1 Schematic representation of a ribosome display selection round. (Amstutz, 2001)

What Can We Do?

With years of experience in the field of in vitro display, Creative Biolabs has helped many customers obtain antibodies/proteins with high affinity and high stability against the target of interest, and won extensive trusts and praises. Leveraging our powerful ribosome display technology and rich expertise in applications of ribosome display, we provide a comprehensive solution for directed evolution of protein stability and affinity, from ribosome display library construction, high-affinity binder screening, to binder evolution with special selection pressure.

Features and Advantages


Creative Biolabs is committed to offering the top-ranking and comprehensive services of optimizing protein affinity and stability for global clients to meet their special purposes. For more information and inquiries about Creative Biolabs' protein evolution services by ribosome display, please do not hesitate to contact us.

References

  1. Hanes, J.; et al. Picomolar affinity antibodies from a fully synthetic naïve library selected and evolved by ribosome display. Nat Biotechnol. 2000, 18:1287-1292.
  2. Jermutus, L.; et al. Tailoring in vitro evolution for protein affinity or stability. Proc Natl Acad Sci USA. 2001, 98:75-80.
  3. Amstutz, P.; et al. In vitro display technologies: Novel developments and applications. Current Opinion in Biotechnology. 2001, 12(4):400-405.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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