Integrative (Hybrid) Modeling

Integrative (hybrid) modeling presents a new emerging concept, which covers the current trends in molecular and cellular structural biology. This is because it provides new methods and recent scientific results for observing and validating structural models of large cellular machines. Creative Biolabs’s integration method is achieved by combining different analysis methods, such as X-ray crystallography, nuclear magnetic resonance spectroscopy, 3DEM, small angle scattering (SAS), FRET, etc., which allows us to deliver thorough protein structure characterization reports for our world-wide customers.

Integrative Modeling Platform

Establishing a structural model of biological systems consistent with countless available data is one of the key challenges of biology. Importantly, simulating the structure and dynamics of macromolecules provides insight into how biological systems work, develop, possibly control, and even design. Based on the integrated modeling platform, Creative Biolabs’s professional bioinformatics team small-angle to providing a comprehensive range of integrated modeling analysis services. Our integrated modeling platform mainly includes but limited to:

X-ray Crystallography

X-ray crystallography is a common technique for determining the three-dimensional structure of proteins, and most of all protein structures (about 85%) have been elucidated using X-ray crystallography. In addition, the requirement for a pure, homogeneous and stable protein solution in the crystallization makes X-ray crystallography be useful in other areas of protein research.

NMR Spectroscopy

NMR spectroscopy is a powerful tool for obtaining information about the structure and dynamics of proteins, which involves the principles of quantum mechanics. Their measurements provide a three-dimensional molecular model of how atoms are chemically linked, their distance in space, and their velocity relative to each other.

Small Angle Scattering (SAS)

SAS is a powerful scattering technique that provides information on the size, shape and orientation of protein structures. The structure of the study sample is typically about range from 0.5 nm to a few 100 nm. The SAS test sample format can be either a homogenous sample, such as a mixture and a liquid, or an anisotropic sample, such as a quasicrystal. The most important feature of SAS is that it can be used to analyze the internal structure of an unordered system.

In addition to the methods featured above, we have also built several techniques to maximize "integration" or "hybrid" modeling. These experimental techniques are short outlined here for clarity. If you need more details, please contact us.

These methods can include:

Electron microscopy (EM) (2D class averages or 3D maps)
Foot-printing
Immunoprecipitation pull-down
Cysteine cross-linking
Chemical cross-linking
FRET spectroscopy
Proteomics

Advantages of Integration Methods

Synergies between input data minimize the disadvantages of incoherent data sets.
Each individual piece of data contains little structural information, but by fitting the model to all data derived from independent experiments at the same time, the degeneracy of the structure suitable for the data can be significantly reduced.
Instead of generating only one structure, this approach has the potential to produce all structures that are consistent with the data.
Analysis of the structure allows us to estimate the accuracy of the data and structure.
This method can make the structure determination process more efficient by indicating which measurement will be the most informative.

Creative Biolabs has been working in the field of antibody & protein engineering for more than a decade, and our scientists are fully confident of completing the most challenging protein structure modeling projects. For additional details on our integrative modeling services, please for free to contact us.

Reference

Sonia, W.; et al. The Clinical Trial Landscape for Integrative (Hybrid) Modeling Therapies. Journal of Clinical Medicine. 2019, 8(3).

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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