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Magic™ Knockout Human HLA-A A-431 Cell Line (CAT#: S01YF-1022-KX642)

Datasheet
This product is a knockout Human HLA-A A-431 stable cell line.

Product Information

  • Target Protein
  • HLA-A
  • Target Protein Species
  • Human
  • Host Cell Type
  • A431
  • Target Classification
  • HLA
  • Target Family
  • HLA Family
  • Target Research Area
  • Ocular Research
  • Related Diseases
  • Severe Cutaneous Adverse Reaction; Birdshot Chorioretinopathy

Product Properties

  • Stability
  • < 20 passages
  • Mycoplasma Testing
  • Negative
  • Biosafety Level
  • Level 1
  • Activity
  • Yes
  • Quantity
  • 1x10⁶ cells
  • Form
  • Frozen cells
  • Culture Medium
  • DMEM, 10% FBS
  • Selective Antibiotic(s)
  • Regular antibiotics active against mycoplasmas, bacteria and fungi.
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Incubation
  • 37°C with 5% CO₂
  • Applications
  • WB
  • Application Notes
  • Cells were plated in a 384-well plate and incubated overnight at 37°C and 5% CO₂ to allow the cells to attach and grow. Cells were then stimulated with a control for high-throughput drugs screening andfunctional assays.
  • Use Restrictions
  • These cells are distributed for research use only.
  • Shipping
  • Dry ice
  • Storage
  • Liquid nitrogen

Target

  • Full Name
  • Major histocompatibility complex, class I, A
  • Introduction
  • HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen so that they can be recognized by cytotoxic T cells. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. More than 6000 HLA-A alleles have been described. The HLA system plays an important role in the occurrence and outcome of infectious diseases, including those caused by the malaria parasite, the human immunodeficiency virus (HIV), and the severe acute respiratory syndrome coronavirus (SARS-CoV). The structural spike and the nucleocapsid proteins of the novel coronavirus SARS-CoV-2, which causes coronavirus disease 2019 (COVID-19), are reported to contain multiple Class I epitopes with predicted HLA restrictions. Individual HLA genetic variation may help explain different immune responses to a virus across a population.
  • Alternative Names
  • HLA-A; HLAA; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; MHC class I antigen HLA-A heavy chain; leukocyte antigen class I-A; Major histocompatibility complex, class I, A

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