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Magic™ Membrane Protein Bacterial nhaA (Na(+):H(+) antiporter NhaA) (CAT#: MP0026F)

Datasheet
Na+/H+ antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 2 H+ per Na+. Can mediate sodium uptake when a transmembrane pH gradient is applied. Active at alkaline pH. Activity is strongly down-regulated below pH 6.5.

Product Specifications

  • Host Species
  • Bacterial
  • Target Protein
  • nhaA
  • Protein Length
  • Full Length
  • Protein Class
  • Transporter
  • Molecular Weight
  • 42 kDa
  • TMD
  • 12
  • Sequence
  • MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLETPVQLRVGSLEINKNML
    LWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAIGGMIVPALLYLAFNYADP
    ITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALAIIDDLGAIIIIALFYTNDLS
    MASLGVAAVAIAVLAVLNLCGARRTGVYILVGVVLWTAVLKSGVHATLAGVIVGFFIPLK
    EKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSLQGVTLDGLTSILPLGIIAGLLIGK
    PLGISLFCWLALRLKLAHLPEGTTYQQIMVVGILCGIGFTMSIFIASLAFGSVDPELINW
    AKLGILVGSISSAVIGYSWLRVRLRPSV

Product Description

  • Activity
  • Validated
  • Application
  • Screening & display technologies
  • Expression Systems
  • Cell-free expression system in the presence of lipid vesicles
  • Tag
  • Histidine tag fused to the N-terminal end of the protein
  • Protein Format
  • Proteoliposome
  • Purification
  • Sucrose gradient
  • Purity
  • >40% by SDS-Page and Coomassie Blue staining
  • Buffer
  • Tris 50mM, pH 7.5

Target

  • Target Protein
  • nhaA
  • Full Name
  • Na(+):H(+) antiporter NhaA
  • Introduction
  • nhaA P2 promoter is RpoS-regulated, but H-NS, Na(+)- and NhaR-independent. [More information is available at EcoGene: EG10652]. NhaA is a sodium ion/proton antiporter that uses the proton electrochemical gradient to expel sodium ions from the cytoplasm and functions primarily in the adaptation to high salinity at alkaline pH.
  • Alternative Names
  • ant, antA, ECK0020, hyc

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