Magic™ Membrane Protein Human SHH (Sonic hedgehog signaling molecule) for Antibody Discovery (CAT#: MP1259J)

Name: Magic™ Membrane Protein Human SHH (Sonic hedgehog signaling molecule) for Antibody Discovery
SKU: MP1259J
Rating: 5 (1 reviews)

Datasheet

This product is a 47.2 kDa Human SHH membrane protein expressed in HEK293T. The protein is for research use only and is not approved for use in humans or in clinical diagnosis.

Product Specifications

Host Species
Human

Target Protein
SHH

Protein Length
Full-length

Protein Class
Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane

Molecular Weight
47.2 kDa

Sequence
MGEMLLLARCLLLVLVSSLLVCSGLACGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKIS
RNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEE
SLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSATVHL
EQGGTKLVKDLSPGDRVLAADDQGRLLYSDFLTFLDRDDGAKKVFYVIETREPRERLLLTAAHLLFVAPH
NDSATGEPEASSGSGPPSGGALGPRALFASRVRPGQRVYVVAERDGDRRLLPAAVHSVTLSEEAAGAYAP
LTAQGTILINRVLASCYAVIEEHSWAHRAFAPFRLAHALLAALAPARTDRGGDSGGGDRGGGGGRVALTA
PGAADAPGAGATAGIHWYSQLLYQIGTWLLDSEALHPLGMAVKSS

Product Description

Expression Systems
HEK293T

Tag
C-Myc/DDK

Purification
Anti-DDK affinity column followed by conventional chromatography steps

Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining

Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Target

Target Protein
SHH

Full Name
Sonic hedgehog signaling molecule

Introduction
This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.

Alternative Names
HHG1; HLP3; HPE3; MCOPCB5; ShhNC; SMMCI; TPT; TPTPS; sonic hedgehog homolog; shh unprocessed N-terminal signaling and C-terminal autoprocessing domains

Gene ID
6469

UniProt ID
Q15465

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