Creative Biolabs offers the unparalleled mass sequencing service to profile the B cell receptor (BCR) and T cell receptor (TCR) repertoires. Equipped with world-leading technology platforms and professional scientific staff in the field of immunology, Creative Biolabs can provide high-grade service of quantitative profiling of immune repertoires for small lymphocytes.

Technical Background to Study Small Lymphocytes

Emerging high-throughput next-generation sequencing (NGS) methods for the analysis of the complex structure of T cell receptors and B cell receptors repertoires give a powerful impulse to adaptive immunity studies. These methods allowed for the deep and, to a certain extent, quantitative analysis of the clonotypic composition of BCR and TCR diversity. This enables researchers to further understand the immune repertoires in health and disease, both at the general scale and in diverse lymphocyte subpopulations. Many different technical approaches have been developed to prepare immune repertoire libraries and correct accumulated PCR and sequencing errors. However, essential technical obstacles for performing a truly quantitative analysis still exist. Specifically, it remains challenging to obtain comprehensive information on the clonal composition of limited small lymphocyte populations, such as antigen (Ag)-specific, tissue-resident cell subsets, microdissection, or fine needle aspirates. Thus, a powerful approach is required to preserve the original clonotypic composition of immune repertoire.

Quantitative Immune Repertoires Profiling for Small Lymphocytes in Creative Biolabs

To achieve the best high-throughput sequencing capacity, Creative Biolabs has integrated the powerful NGS technology and many other specific techniques into our unique Magic™ platform. Creative Biolabs has developed a robust high-yield NGS approach based on unique molecular identifiers (UMIs), which allows qualitative and quantitative profiling of immune repertoires on the clonal composition of small lymphocytes. The process consists of cell preparation, PCR amplification, sequencing on our Magic™ platform and data analysis. Above all, we employed barcoding of each cDNA molecule using UMIs. This strategy minimizes quantitative biases associated with uneven amplification efficiency for different Ag receptor genes.

Key Advantages

• Qualitative and quantitative BCR/TCR profiling
• Special for small lymphocyte populations, such as Ag-specific, tissue-resident cell subsets, microdissection, or fine needle aspirates.
• Based on UMIs
• Low amount of sample input

Creative Biolabs has established the high-throughput Magic™ platform for large-scale sequencing services. With years of research and development experience in the field of immunology, Creative Biolabs has accumulated sufficient experience in quantitative profiling of BCR/TCR repertoires for small lymphocytes. We are pleased to offer the best service with the most accurate results for our global customers.

Please contact us for more information and a detailed quote.

FAQ

1. What is quantitative immune repertoire profiling for small lymphocytes

   Quantitative immune repertoire profiling analyzes the diversity and abundance of lymphocyte receptors, such as T-cell and B-cell receptors. This profiling helps in understanding the immune responses mediated by small lymphocytes. By quantifying receptor diversity, the profiling assesses the clonal expansion and variability of lymphocyte populations in response to infections or diseases like cancer.

2. How does quantitative immune repertoire profiling differ from qualitative methods

   While qualitative profiling identifies the types of receptors present, quantitative profiling goes further by assessing the frequency and clonal expansion of lymphocyte receptors. This provides a more detailed understanding of the immune system's activity, particularly in small lymphocyte populations, allowing for precise tracking of immune responses and potential biomarkers.

3. What technologies are used for quantitative immune repertoire profiling

   Technologies such as next-generation sequencing (NGS) and single-cell sequencing are commonly used for quantitative immune repertoire profiling. These methods allow high-throughput analysis of lymphocyte receptor sequences, providing detailed insights into the immune landscape by quantifying TCR or BCR diversity, clonal expansion, and overall receptor usage in small lymphocytes.

4. Can quantitative immune repertoire profiling be applied to cancer research

   In cancer research, this profiling reveals the immune system's role in tumor recognition and elimination. It helps in identifying T-cell clones targeting tumor antigens, providing essential data for immunotherapy development. Tracking the diversity of small lymphocyte populations can reveal how well the immune system is responding to the tumor or whether it is suppressed.

5. How is data from quantitative immune repertoire profiling analyzed

   Data from quantitative immune repertoire profiling are analyzed using advanced bioinformatics tools that process large sequencing datasets. These tools assess receptor diversity, clonal expansion, and sequence similarity. The analysis allows researchers to identify dominant lymphocyte populations and track changes in immune responses over time, making it essential for clinical decision-making.

6. What are the challenges of quantitative immune repertoire profiling

   Challenges include managing large datasets generated from sequencing and ensuring sample quality. There is also the complexity of interpreting immune diversity, especially when rare lymphocyte populations are involved. Overcoming these hurdles requires robust bioinformatics and a deep understanding of immune system dynamics, particularly for small lymphocyte populations.

7. How can quantitative immune repertoire profiling inform immunotherapy strategies

   Quantitative profiling identifies specific lymphocyte clones that recognize tumor or pathogenic antigens, enabling targeted immunotherapy strategies. By understanding the immune repertoire's composition, researchers can enhance T-cell responses, design personalized vaccines, or develop therapies that boost the immune system's ability to recognize and attack cancer cells or pathogens.

8. What are the future directions for quantitative immune repertoire profiling

   Future directions include integrating multi-omics approaches to combine immune repertoire profiling with transcriptomics and proteomics. This will provide a more comprehensive view of lymphocyte functionality. Advances in single-cell technologies will also allow for more precise characterization of individual lymphocyte responses, improving the understanding of immune dynamics in health and disease.

Resources

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