Creative Biolabs offers the unparalleled mass sequencing service to analyze the B cell receptors (BCR) repertoires on our cutting-edge Magic™ platform. Scientists of Creative Biolabs can also provide unbiased BCR repertoire profiling by high-throughput genome-wide translocation sequencing-adapted repertoire sequencing. We are proud to offer high-throughput, high­fidelity and high­quality sequencing data for our customers all over the world.

Unbiased Analysis Is Urgently Needed in BCR Profiling

The adaptive immune system drives the immune response via specific hypervariable B cell receptor (BCR) molecules. Amazing diversity makes the immune system the most effective system to fight against a broad scope of disease-causing pathogens. The repertoire is generated by a complex series of genetic events. Developing B lymphocytes undergo V(D)J recombination to assemble germline V, D, and J gene segments into exons, which encode the antigen-binding variable region of Ig heavy (H) and light (L) chains. High-throughput next-generation sequencing (NGS) has already had a profound effect on our knowledge of the immune systems physiology. NGS opened an amazing opportunity to perform deep analysis and comparative studies of the BCR repertoire. It has revolutionized this field by allowing massive analysis of BCR repertoires from individual blood samples. It is becoming a powerful tool for deep BCR profiling. BCR mRNA molecules include highly homologous variants. Hence, performing deep and unbiased analysis of millions of BCR mRNA molecules is quite challenging, due to the accumulation of PCR and sequencing bias. Multiple sampling bottlenecks, PCR biases, and cross-contamination at different stages hinder the way of researchers to get the deep, clear, and consistent data. Obtaining high-throughput, high­fidelity and high­quality sequencing data is a critical challenge in BCR repertoire profiling, but it is essential for biomarker analysis and the generation of diagnostic and therapeutic antibodies.

Fig.1 Process of generating a diverse B cell repertoire (Hou et al. 2016).

Unbiased BCR Repertoire Profiling in Creative Biolabs

To achieve these ends, Creative Biolabs developed a powerful method for unbiased BCR repertoire profiling based our Magic™ platform. We have adopted an unbiased and sensitive method, referred to as high-throughput genome-wide translocation sequencing-adapted repertoire sequencing. Our advanced pipeline for immune repertoire analysis can identify the vast majority of IgH and IgL V(D)J exons, including their unique CDR3 by using specific J primers. We developed cell barcodes for PCR contamination detection and error correction, including error correction of both RT­PCR and base­calling sequencing errors as well as accurate assessment of clonal proportions.

Key Advantages of Unbiased BCR Repertoire Profiling

• Unbiased and sensitive
• High-throughput genome-wide translocation sequencing-adapted repertoire sequencing
• Using specific J primers
• High throughput, high fidelity, and high quality

Diverse Species of Our Unbiased BCR Repertoire Profiling

• Feline Immunoglobulin Repertoire Profiling
• Zebrafish Antibody Repertoire Analysis
• Alpaca BCR Repertoire Profiling
• Canine BCR Repertoire Profiling
• Rabbit BCR Repertoire Profiling
• Swine BCR Repertoire Profiling

Applications of Our Unbiased BCR Repertoire Profiling

• B Cell Clones Identification
• Novel Alleles Identification
• SHM Patterns Analysis

Equipped with world-leading technology platforms and professional scientific staff in immunology, scientists of Creative Biolabs have performed over hundreds of BCR repertoire sequencing projects and accumulated sufficient experiences. We can provide unbiased BCR repertoire profiling with high-throughput, high­fidelity, and high-quality data. We are pleased to offer the best service with the most accurate results for our global customers.

Please contact us for more information and a detailed quote.

FAQ

1. What is unbiased BCR repertoire profiling?

   Unbiased BCR repertoire profiling refers to a comprehensive method of analyzing the full diversity of B cell receptors (BCRs) without introducing any biases during the process. This technique allows for the accurate identification and quantification of all BCR variants present within a sample, providing critical insights into the adaptive immune system's response to infections, vaccinations, or autoimmune disorders.

2. How is unbiased BCR repertoire profiling different from traditional methods?

   Traditional BCR profiling methods often involve amplification techniques that may introduce bias, favoring certain receptor sequences over others. Unbiased BCR repertoire profiling avoids these biases by using advanced next-generation sequencing (NGS) and bioinformatic tools, ensuring that the full range of BCR diversity is captured. This leads to more accurate data on immune responses and clonal expansions.

3. What are the applications of unbiased BCR repertoire profiling?

   Unbiased BCR repertoire profiling has wide applications in immunology, oncology, vaccine development, and autoimmune disease research. It is used to study immune responses to pathogens, track B cell clonal evolution, identify disease-associated B cell clones, and monitor immune reconstitution following therapies like bone marrow transplants. It is also valuable in developing personalized medicine approaches for cancer and infectious diseases.

4. What challenges exist in unbiased BCR repertoire profiling?

   Challenges in unbiased BCR repertoire profiling include ensuring complete coverage of the BCR diversity in a sample, managing the vast amount of data generated by next-generation sequencing, and accurately interpreting complex B cell clonal dynamics. Additionally, technical issues such as PCR biases, sequencing errors, and the need for robust bioinformatics pipelines can complicate data analysis and interpretation.

5. How does unbiased BCR repertoire profiling help in cancer research?

   Unbiased BCR repertoire profiling is valuable in cancer research for tracking the evolution of B cell clones and understanding tumor-immune interactions. By identifying specific B cell clones associated with tumor antigens, researchers can monitor immune responses to immunotherapies and detect minimal residual disease (MRD). This helps in predicting patient outcomes and guiding personalized treatment strategies.

6. How is data generated from unbiased BCR repertoire profiling analyzed?

   Data from unbiased BCR repertoire profiling is analyzed using specialized bioinformatics tools that process high-throughput sequencing data. These tools align the BCR sequences, identify unique clonotypes, and quantify clonal diversity. Further analysis includes examining somatic hypermutation rates, isotype distribution, and clonal expansion patterns. Visualization techniques, such as clonal phylogenetic trees, are often employed to interpret the data.

7. How can unbiased BCR repertoire profiling be used in monitoring immune therapies?

   Unbiased BCR repertoire profiling is a critical tool for monitoring immune reconstitution following therapies such as bone marrow transplants or CAR-T cell therapy. By tracking the diversity and clonal expansion of B cells, clinicians can assess the recovery of the immune system and detect any potential issues, such as graft-versus-host disease (GVHD) or infection susceptibility, thereby improving patient management.

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