Photosynthetic Bacteria based Membrane Protein Expression Service

Creative Biolabs constantly strives to provide unparalleled membrane protein expression service using a variety of systems, particularly photosynthetic bacteria cells. Our scientists have accumulated a wealth of experience after long-term exploration, and successfully established and commercialized a world-leading Magic™ Membrane Protein Production Platform. We are determined to assist our worldwide clients to facilitate their research progress.

Introduction

Photosynthetic bacteria are capable of converting light energy into chemical energy through photosynthesis. Unlike plants, which utilize chlorophyll-based systems in chloroplasts, photosynthetic bacteria have specialized structures called chromatophores or thylakoids where photosynthesis occurs. These structures are embedded with a diverse array of membrane proteins essential for capturing light and converting it into energy. In photosynthetic bacteria, the expression of membrane proteins is critical for the photosynthetic process. These proteins include reaction centers, light-harvesting complexes, and electron transport chains, which collaborate to achieve efficient energy conversion.

Principle

Photosynthetic bacteria hold a featured inducible intracytoplasmic membrane (ICM). Under conditions of light and/or lowered oxygen, the membrane surface of the native organism increases significantly when the ICM is elaborated as an invagination of the cytoplasmic membrane. Moreover, such environmental stimulations induce the synthesis of the photosynthetic apparatus. The new ICM in the native organism sequesters these complexes that are composed of transmembrane polypeptides and their associated hydrophobic redox and energy transfer cofactors. Upon cell splitting, the ICM breaks apart from the host's cytoplasmic membrane becoming sealed "inside-out" particles that can be easily isolated by differential centrifugation through their size. With the development of biologics, large quantities of active membrane proteins can be obtained. As such, photosynthetic bacteria system exerts great potential for both biotechnology and pharmaceutical industries.

Photosynthetic Bioprocesses Fig.1 Membrane protein in photosynthetic bacteria.¹

Advantages of Our Photosynthetic Bacteria based Membrane Protein Expression Service

Lower production costs
Ease of purification
High yields (0.5 mg/L to as high as 20 mg/L)
Simultaneous production and sequestration of proteins are available

Creative Biolabs has extensive expertise in membrane protein expression. Our one-stop service can offer customers the most flexible options and most reliable outcomes. Please feel free to contact us for more information.

We also provide other cell-based membrane protein expression systems, including:

Reference

Stephens, Sheida, Radhakrishnan Mahadevan, and D. Grant Allen. "Engineering photosynthetic bioprocesses for sustainable chemical production: a review." Frontiers in Bioengineering and Biotechnology 8 (2021): 610723. Distributed under Open Access license CC BY 4.0, without modification.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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