iPSC Reprogramming Service for Adipose-Derived Stem Cells

Overview of Human Adipose-Derived Stem Cell

Approximately 0.4 million liposuction surgeries are conducted in the USA each year and yield abundant lipoaspirate tissue which is usually discarded. In recent research, easily accessible adipose tissue is demonstrated to contain a rich source of adult stem cells with the capacity for self-renewal and multilineage differentiation. Human adipose-derived stem cells (hADSCs or hASCs), also known as preadipocytes, have shown their veritable potential in multiple-direction research such as stem cell research, regenerative medical research, and tissue engineering. hADSCs-derived induced pluripotent stem cells (iPSCs) have been generated from easily accessed adipose of patients, which speeds up disease research and drug discovery and depicts a bright future for person-specific treatment strategy.

Benefits of hADSCs Reprogramming

Many different types of human cells, including skin fibroblasts, urine cells, and blood stem cells, may currently be reprogrammed to generate iPSCs. hADSCs become an attractive initial material for reprogramming with the following benefits.

• A minimally invasive procedure is involved in the collection and harvesting of hADSCs. Adipose isolation causes no or little influence on donor health.
• Millions to billions of cells could be isolated from adipose tissue.
• Matured in vitro hADSC culture and expansion platform has been well-established in recent decades.
• hADSCs have less methylation at the genome compared with terminally differentiated cells such as fibroblasts, leading to a higher successful rate in reprogramming.

Services at Creative Biolabs

Reprogramming Workflow for hADSCs

1. Adipose tissue is collected, washed, and digested for the following isolation of hADSCs. Enriched hADSCs are expanded to generate enough cell numbers for reprogramming initiation.

2. Multiple methods such as the Sendai virus or reprogramming factor-contained vectors help the reprogramming of hADSCs to iPSCs. Cells undergo morphology change during the induction.

3. Clones with typical characteristics of iPSCs are picked up to generate single-cell clones for further expansion and cryopreservation.

4. Quality control like immunofluorescence staining of pluripotent markers and karyotype are conducted for each single cell clone that will be delivered.

Fig.1 Reprogramming of hADSCs yields well-qualified iPSC clones.¹

Custom hADSCs Reprogramming Service

The first documented instance of iPSCs is considered a watershed moment in biological science. Like traditional fibroblast-derived iPSCs, hADSCs-derived iPSCs also provide a promising tool for disease modeling, new drug discovery, and regenerative research, regarding its unique advantages of easy accessibility, isolation, and expansion. Most importantly, it is compatible with nearly all the reprogramming methods. In combination with other services such as high-throughput screening, organoids, sequencing, and in vitro differentiation platform, hADSCs-derived iPSCs are an attractive candidate for your important research.

Creative Biolabs has established a high-efficiency iPSC reprogramming platform and provides well-qualified iPSC single-cell clones to our worldwide clients to facilitate their research. Nowadays, we realize the efficient method to reprogram hADSCs into iPSCs and refine this method to reach a high success rate. Don't hesitate to contact us for a tailored and optimal strategy for your research goal.

Features of Our Services

Our service is recognized for its comprehensive approach that guarantees quality and efficiency. Specific advantages are shown:

• Reprogramming Technology - Our service utilizes a proprietary reprogramming technology that has been developed in-house. This technology ensures superior efficiency and reliability in converting ADSCs into iPSCs, offering a competitive advantage in iPSC generation.
• Enhanced Pluripotency Maintenance - Our reprogramming process includes proprietary culture conditions and growth factors optimized for maintaining the pluripotency of iPSCs derived from adipose tissue. This results in iPSC lines that exhibit enhanced self-renewal capacity and differentiation potential, making them ideal for various downstream applications.
• Disease Modeling Expertise - With our deep expertise in disease modeling using iPSCs, we offer specialized reprogramming services for generating disease-specific iPSC lines from adipose-derived stem cells. Whether it's cardiovascular diseases, metabolic disorders, or rare genetic conditions, we have the knowledge and resources to generate iPSC models that faithfully recapitulate disease phenotypes.
• Functional Characterization Assays - In addition to standard pluripotency assays, we offer a comprehensive suite of functional characterization assays to assess the quality and utility of generated iPSC lines. This includes differentiation assays, functional genomics analysis, and iPSC based drug screening platforms tailored to specific research or therapeutic objectives.

FAQs

• Q: Can this service work with a small number of initial cells?
  A: Yes, we can certainly conduct the reprogramming process with a small number of cells. However, it's worth noting that a higher number of initial cells increases the chances and efficiency of successful reprogramming.
• Q: What is the success rate of the reprogramming service?
  A: While the success rate can vary depending on the condition of the initial cell templates, our experienced team and advanced methods ensure high success rates, typically achieving reprogramming efficiency above industry standards.
• Q: How long does the whole reprogramming process take typically?
  A: The reprogramming process varies in length depending on the specific properties of the cells involved, but it typically takes around 3-4 weeks for visible iPSC colonies to appear. However, it may take an additional several weeks for further validation and characterization of the derived iPSCs.
• Q: What is the scalability of your iPSC reprogramming service for adipose-derived stem cells? Can you accommodate large-scale projects or high-throughput screening applications?
  A: Yes, our iPSC reprogramming service is scalable to accommodate projects of various scales, including large-scale initiatives and high-throughput screening applications. We possess the infrastructure, resources, and expertise to efficiently scale up operations while maintaining the quality and consistency of iPSC generation to meet your project's demands.
• Q: How do you ensure traceability and documentation of iPSC reprogramming processes and experimental data?
  A: We maintain meticulous traceability and documentation throughout the iPSC reprogramming process and experimental workflows. This includes comprehensive record-keeping of protocols, procedures, and experimental parameters, as well as data management systems to track sample identities, experimental conditions, and results, ensuring transparency, reproducibility, and compliance with regulatory standards.

Scientific Resources

iPSC Reprogramming

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Reference

1. Qu, Xinjian, et al. "Induced pluripotent stem cells generated from human adipose-derived stem cells using a non-viral polycistronic plasmid in feeder-free conditions." PloS one 7.10 (2012): e48161.

For Research Use Only. Not For Clinical Use.