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Neuronal Cells Differentiation from iPSC

Overview Service Features Published Data FAQs Scientific Resources Related Services

Creative Biolabs offers the most advanced and comprehensive iPSC services for our customers all over the world. With years of experience, scientists at Creative Biolabs now are able to generate the neuronal cells via our iPSC differentiation technology.

Introduction of iPSC-derived Neuronal Cells

With the unlimited proliferative capacity and extensive differentiation potential, human pluripotent stem cells (hPSCs) present distinct advantages than adult stem cells. In order to avoid the ethical issues, the application of human embryonic stem cell (hES cells) is replaced by human induced pluripotent stem cells (hiPSCs). which can be generated from various adult somatic cell types via the introduction of four specific transcription factors: OCT3/4, SOX2, KLF4, and c-MYC. However, these hiPSCs cannot be used directly into patients because of the tumorigenic potential. In this case, a differentiation protocol is an essential prerequisite to direct hiPSCs into specific cell types. As such, the neural differentiation of hiPSCs enables a promising cell source for both regenerative medicine and cell therapy.

Neuronal cells immunostaining after two weeks of differentiation.Fig.1 Neuronal cells immunostaining after two weeks of differentiation.

Services at Creative Biolabs

Neural Differentiation of hiPSCs

The neuronal cell differentiation from iPSC offers great promise to both basic research and clinical applications. Previously, the traditional technologies utilized to initiate neural differentiation of hiPSCs are embryoid body formation and co-cultivation with stromal cell lines. However, there are a series of disadvantages, including time-consuming preparation, low efficiency, complicated process, and variability results, largely limit their applications. In this case, now Creative Biolabs has built the high-efficient neural differentiation method based on chemically defined culture milieu and small molecular inducers. The principle of our protocol relies on the interplay of activation and inhibition of multiple developmental signaling pathways. The small molecules enable the modulation of the key developmental signaling pathways thus regulate neural differentiation of hiPSCs. Now we focus on directing hiPSCs into four major specific neuronal sublineages which are serotonergic, cholinergic/motor, dopaminergic, and serotonergic neurons. In summary, we are able to get various neuronal cell types based on our special synthetic small molecules, and more specifically potent small molecules are under development for precise control of neurogenesis.

The generation of neurons from hiPSCs.Fig.2 The generation of neurons from hiPSCs.

Features of Small Molecules

  • Cost effective.
  • Stable and reduce experimental variability.
  • Rapid, reversible, and tuneable biological effects.

Applications of iPSC-derived Neuronal Cells

  • In vitro study for neural development and neurological diseases mechanisms.
  • Drug development, screening, and toxicology assays.
  • Therapeutic targets identification.
  • In vitro modeling of human neurological diseases.
  • Autologous cell sources for therapy of various neurological diseases.
  • Autologous cell therapy for regenerative medicine.

With our well-established iPSC differentiation technology, Creative Biolabs now can generate iPSC-derived neuronal cells for our clients. Based on our extensive experience and advanced platform, our service is built to meet the customer's project and specific requirements. Creative Biolabs also provide other services regarding iPSC differentiation, please feel free to contact us if you are interested in them.

Features of Our Services

  • Custom differentiation
  • Validation data
  • A diverse range of neuronal subtypes such as cortical neurons, motor neurons, dopaminergic neurons, and more
  • High-quality, reliable
  • Capable of handling large-scale projects

Published Data

1Below are the findings presented in the article related to neuronal cell differentiation from iPSC.

Minami Hiranuma, et al. studied the expression of sensory neuron-related proteins and genes as well as drug responses by multi-electrode array (MEA) to analyze sensory neuron properties and functions. They confirmed the expression of sensory neuron-related genes and proteins by real-time PCR and immunocytochemistry (ICC) in order to characterize human iPSC-derived sensory neurons.

As shown in the figure below, ICC showed the expression of TUBB3 (a marker for mature neurons), Peripherin (a marker for peripheral neurons) and Brn3a (a marker for sensory neurons) at 14 DIV. In conclusion, hiPSC-derived sensory neurons express sensory neuron-related genes and proteins.

Expression and morphology of sensory neuron-associated proteins in hiPSC-derived sensory neurons. (Hiranuma, Minami, et al., 2024)Fig. 3 Expression and morphology of sensory neuron-associated proteins in hiPSC-derived sensory neurons.2

FAQs

  • Q: What is the application of your neuronal cell products in scientific research?
    A: Our differentiated neuronal cells from iPSCs have wide-ranging applications in various fields of scientific research. This includes studying the pathophysiology of neurodegenerative diseases like Alzheimer's and Parkinson's disease, in drug discovery and toxicity testing, and in developing therapeutics for numerous neurological disorders. They can also be used to model different neurological diseases to understand their progression and mechanism.
  • Q: What type of neurons can your service provide?
    A: Our differentiation service can generate various types of neuronal cells from iPSCs, including cortical neurons, dopaminergic neurons, motor neurons, and sensory neurons. The choice of the specific neuronal cell type is usually determined by the needs of the client's research objectives.
  • Q: Is the pricing for your service flexible?
    A: Yes. Our pricing is designed to be flexible to accommodate different research budgets. It is influenced by several factors such as the type of neurons needed, the quantity, and any additional services required. We are always willing to work with the client to meet their budgetary needs.
  • Q: Can you assist with the integration of differentiated neuronal cells into 3D culture systems or organoid models?
    A: Certainly. We offer expertise in integrating differentiated neuronal cells into various 3D culture systems, including organoids, spheroids, and microfluidic devices. Our team can provide guidance on experimental design, culture protocols, and imaging techniques to facilitate the integration of neuronal cells into complex 3D models. Whether you're interested in studying neuronal development, disease modeling, or drug screening in 3D culture, we can assist you in optimizing your experimental approach.

Scientific Resources

References

  1. Yap, M.S. (2015). “Neural differentiation of human pluripotent stem cells for nontherapeutic applications: toxicology, pharmacology, and in vitro disease modeling.” Stem Cells International 2015, 105172.
  2. Hiranuma, Minami, et al. "Characterization of human iPSC-derived sensory neurons and their functional assessment using multi electrode array." Scientific Reports 14.1 (2024): 6011.

For Research Use Only. Not For Clinical Use.