With many years of experience and advanced technologies, Creative Biolabs has developed an innovative STEMOD™ platform to provide high content screening (HCS) services for our global customers. Our optimized systems will help you quickly get satisfactory results and contribute greatly to the success of your stem cell-based programs.
High-content screening is an advanced approach that can be used in many fields, such as drug discovery and biological research, to identify substances that alter the phenotype of a cell in the desired manner. It is a type of phenotypic screen conducted in cells to analyze complex cellular/organoid events and visualize the relevant phenotypes.
Recently, high-content screening has been used in drug discovery and fundamental research, from identifying hit compounds in primary drug screening and profiling cellular responses to specific perturbations. This highly multiplexed bioassay technology uses diverse techniques such as live-cell imaging, whole-animal imaging, spheroid cultures, etc., and it can be applied for the analysis of neuronal damage, T cell killing function, stem cell differentiation, nerve synapse length, nerve synapse growth, immune cell infiltration and so on.
Fig.1 The role of HCS technology. 1
High content screening and high-content analysis are extremely powerful tools for the quantitative analysis of large volumes of image data. In addition, it can provide novel insight into many complex biological questions. Creative Biolabs provides a range of solutions for high content imaging and analysis and phenotypic screening based on stem cell models. Our dedicated team of specialists has an in-depth understanding of stem cell model-based high content screening and helps you find the best-fit solution for your research requirements. This high-content screening service will advance your research in a diverse range of scientific research areas, including neurology, immuno-oncology, toxicology, stem cell research, etc.
STEMOD™ based high content screening allows visualization of important aspects of cancer initiation and progression, including T cell killing function, tumor cell mobility, invasion, metastasis, and angiogenesis.
STEMOD™ based high content screening can analyze various aspects of neurology, including neuron damage, nerve synapse length, nerve synapse growth, cell migration, proliferation, and apoptosis, immune cell infiltration.
The ability to measure early indicators of toxicity is an essential part of drug discovery. In addition, high content screening is a cost-effective tool to run dedicated toxicity tests on relevant model systems to reduce development costs and increase research efficiency.
High content analysis can be used for analyzing stem cell differentiation efficiently under a large variety of conditions and over long periods.
Creative Biolabs is one of the well-recognized experts who are professionals in high-quality stem cell-based technology and solutions. Scientists at Creative Biolabs are dedicated to providing STEMOD™-based high content screening service to meet our customers' specific requirements. To learn how high-content analysis and screening can advance your research in a selection of areas, please do not hesitate to contact us.
Below are the findings presented in the article related to high content screening for stem cells.
iPSC-derived neurons are increasingly being used for high-content imaging and screening. Elizabeth R. Sharlow et al. developed a miniaturized, feeder-free iPSC-derived human cortical neuron detection system specifically designed to identify NeuN maturity markers from asynchronously maturing "mixed" neuronal cultures expressing the NeuN maturity marker.
Using an imaging platform compatible with high content applications and feeder-free neuronal progenitor cells (NPCs) from healthy human patients, they have further improved the inoculation, processing and culture conditions of iPSC-derived NPCs to minimize neuronal aggregation associated with neuronal maturation. In addition, they developed an imaging-based algorithm for identifying and quantifying individual iPSC-derived cortical neurons from mixed neuronal cultures consisting of large cell aggregates and more dispersed neurons.
This miniaturized detection system could provide a foundation for modeling neurological diseases and improve screening studies based on mature neurons.
Fig. 2 Pilot studies using population- and single cell image-based screening formats.2
References
For Research Use Only. Not For Clinical Use.
