CRM197 protein (VCar-Lsx004)

CAT	Size	Price	Quantity
VCar-Lsx004

CRM197, which is widely used as a carrier protein in conjugate vaccines, was expressed in Corynebacterium diphtheriae, and purified by state-of-art protein purification method such as centrifugation, amonium sulfate precipitation and chromatography, then lyophilized after addition of sucrose.

Datasheet

Specifications

Source

Corynebacterium diphtheriae

Target

Glu52 diptheria toxin antibody

Type

Proteins

Applications

Used for detection of antibodies or as a carrier protein for conjugate vaccines.

Quantity

Before lyophilization, CRM197(aq) was about 5mg/ml in sterile water with 5% sucrose; Vials containing appoximately 5mL of CRM197(aq) were lyophilized.

Purity

>90%

Antigen Information

Introduction

CRM197 is a non-toxic mutant of diphtheria toxin, currently used as a carrier protein for polysaccharides and haptens to make them immunogenic. CRM197 is a genetically detoxified form of diphtheria toxin. A single mutation at position 52, substituting glutamic acid for glycine, causes the ADP-ribosyltransferase activity of the native toxin to be lost. CRM197 is widely used as a carrier protein for conjugate vaccines, because it is genetically detoxified and retains its full complement of lysine amines for conjugation. There is also evidence suggesting that, compared with tetanus toxoid, there is less carrier-induced suppression of the immune response, especially when there are many individual polysaccharides linked to the same carrier protein.

Alternative Names

CRM197; Glu52 diptheria toxin; Cross-reactive materials 197