Liposome based Membrane Protein Reconstitution Service

With a lipid bilayer structure, liposomes are a proper system to mimic cell membranes in many cases. Reconstitution of membrane proteins into liposomes, which forms proteoliposomes, can keep membrane proteins correctly folded with their native structures, activities, and functions. Reconstitution of membrane proteins into liposomes is never an easy task to accomplish, mainly due to the high propensity for membrane proteins to aggregate and the difficulty in the preparation of stable, homogeneous proteoliposomes in desired size. However, scientists in Creative Biolabs have rich knowledge and decades of experience in performing experiments in this particular area. We are professional in proteoliposome preparation from various starting materials, such as intact cells, membrane crude extracts, and membrane proteins already solubilized or reconstituted in micelles, bicelles, nanodiscs, amphipols, or other liposomes.

Membrane Protein Reconstitution in Liposomes

Based on our lipid screening platform, a large variety of lipids either synthesized or extracted from natural sources are available in Creative Biolabs. We can also dope the proteoliposomes with proper types and fractions of charged lipids, modified lipids, and other additives like cholesterols to stabilize the proteoliposomes and maximally keep the membrane proteins’ function in their native form. We can perform comprehensive detergent screening platform with various detergents to select the one that does not cause unfolding, destabilization, aggregation, or conformational change of the target membrane proteins. After the solubilization and purification, we will add a series of lipids to the micellar solution of your membrane proteins followed by removing the detergents via hydrophobic beads binding or dialysis.

Membrane Protein Reconstitution in Liposomes (Creative Biolabs)

Treatment after Membrane Protein Reconstitution in Liposomes

After the formation of proteoliposomes, occasionally further treatment is desired to change the proteoliposome size or change the liposome morphology.

Freeze-thaw
Extrusion
Sonication
Gradient separation of proteoliposomes from empty liposomes

Advantages of Membrane Protein Reconstitution in Liposomes

Complete unilamellarity or multilamellarity
High homogeneity and monodispersity
Desired size
Desired fractions of each component
High purity
High stability

Applications of Membrane Protein Reconstitution in Liposomes

Antibody discovery
Functional studies
Solution-state NMR and solid-state NMR
Drug discovery
Structure studies

In terms of our unparalleled technologies and years of expertise in research and development of biological and biochemical products, Creative Biolabs will greatly facilitate your project success. Our custom service in the reconstitution of membrane proteins into liposomes will provide you high-quality proteoliposomes that are most suitable for your goals. As an alternative, you can also choose our professional custom services in membrane protein solubilization/reconstitution in micelles, nanodiscs, or amphipols. Please feel free to inquire us for a detailed quote.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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