One of the hallmarks of membrane proteins is their hydrophobic transmembrane domains. To keep membrane proteins homogeneously dispersed in aqueous solutions with their native functions intact, it is almost inevitable to apply micelles, cell membrane-like lipid bilayer systems, or polymers to solubilize and stabilize them. Detergent is a common strategy for membrane protein solubilization, which generally leads to the formation of spherical micelles. To achieve the extraction of different membrane proteins with varying properties, detergents screening has been utilized to find a suitable detergent for each specific case. Selection of appropriate detergent is very crucial as misuse of detergent might compromise protein structure, resulting in denatured proteins.
Screening for detergent or micelle-forming lipid to construct micelles solubilizing a membrane protein can be quite a labor- and capital-intensive task. Each detergent or micelle-forming lipid may have very different stabilizing or destabilizing effects on a membrane protein. In most cases, different micelles need to be used for membrane protein extraction from different sources, as well as for different applications. With rich experience in this area and a series of detergents and lipids available, Creative Biolabs has built comprehensive detergent screening platform and lipid screening platform to find the best micelles for our customers' specific membrane protein targets with high purity, stability, and yield.
Working on membrane proteins can be very time-consuming. Creative Biolabs is always ready to help you save your valuable time through our first-class membrane protein synthesis, reconstitution, purification, and characterization services. As an alternative to the micelle system, we also offer services in solubilization/reconstitution of membrane proteins in liposomes, nanodiscs, and amphipols. Please do not hesitate to inquire us for more information.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
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