B Cell Products

Characteristics of B cells

B cells or B lymphocytes arise from hematopoietic stem cells and serve as an important lymphocyte cell type in the mammalian adaptive immune system producing humoral immunity responses. B lymphocytes perform multiple functions, such as producing antibodies, serving as APCs, supporting other mononuclear cells, and directly promoting inflammatory signal pathways.

Fig.1 The role of B cells in COVID-19 infection and re-infection.¹

B Cell Activation

On the one hand, B cells function as APCs to receive protein antigen activation and present protein epitopes on MHC II molecular to the helper T cells, and this mechanism is commonly referred to as T cell-dependent activation of B cells. On the other hand, some non-protein antigens, lipopolysaccharides, and polysaccharides serve as T-independent antigens, and these molecules activate B cells without the need for antigen processing and presentation to T cells. Hence this mechanism is called T Cell-independent activation of B cells.

B Cells Isolation

Generally, there are several approaches used for B cell isolation. Positive selection and negative selection as the two main methods. Positive selection is a method by which B cells are individually isolated and retained for downstream analysis after being targeted or labeled using a B cell isolation kit (positive selection). Negative selection is a method by which other cell types are removed first using a B cell isolation kit (negative selection), leaving B cells behind. In addition, B cell depletion is another method, where a single B cell type is depleted from a cell sample.

The Application Fields of B cells

There are multiple research areas applications for B cells including:

• Multiple myeloma research
• Chronic lymphocytic leukemia research
• Blood cancer research
• Autoimmunity research
• Cancer immunology study
• Infectious diseases study
• Antibody development
• Vaccine development

B Cells at Creative Biolabs

B cells are suitable for a variety of downstream applications. To better help global customers achieve the success of the project, Creative Biolabs offers a variety of ready-for-use, highly pure B cells from whole blood obtained from IRB-consented donors. At the same time, we also customize the appropriate cell types according to the customers' special project needs.

Our wide portfolio of B cell products ensures global customers' variable needs and consistent results in the related projects. The following items are some hot cell products you can refer to find a suitable product for your project. For more defined requirements, please feel free to contact us or send us an inquiry.

Reference

1. Chen, Shiru, et al. "The role of B cells in COVID-19 infection and vaccination." Frontiers in Immunology 13 (2022): 988536.