In Vivo CRS Model Model Development Services

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Cytokine release syndrome (CRS) is a serious and potentially life-threatening complication typically associated with biological drug products. Animal models have been long established to facilitate the study of pathogenesis of the major toxicities of different therapies. However, the mouse models and in vitro human PBMC assays presently in use do not reliably predict CRS in patients. Creative Biolabs offers different kind of in vivo CRS model tools to support your CRS management.

PBMC-engrafted Humanized In Vivo Mouse Model

Humanized mouse models provide a potential translational bridge for the study and prediction of CRS in vivo. Studies have suggested the potential of using immunodeficient mice engrafted with human immune systems to study CRS. PBMC engrafted NSG, NSG-SGM3, and NSG-MHC-DKO mice are frequently used to study cytokine release in response to several immunotherapeutic, including anti-CD3, anti-CD28, anti-thymocyte globulin (ATG), and a TGN1412 analog. In Vivo CRS Models

Engraftment of human PBMC in mice
Flow cytometry analysis
Induction of cytokine release and cytokines quantification
In vitro assay for evaluating cytokine release
Statistical analysis

Donor-dependent In Vivo Mouse Model

Currently, the only widely accepted predictors of CRS are cancer burden and therapeutic dose. Despite this, most pre-clinical assays that evaluate CRS do not incorporate cancer cells and the safety of drug combinations has not been widely explored. A predictive assay that identifies cancer-patient-therapy combinations at risk for developing CRS upfront would improve the safety of immune-oncology drug development. Scientists at Creative Biolabs have developed different in vivo models to evaluate donor-dependent cytokine release in response to single-agent or combination immune-oncology therapies.

LPS-Induced Cytokine Release Model

A massive cytokine release can be experimentally induced in serval ways. Lipopolysaccharide (LPS) is a toxin that is routinely used to activate a systemic inflammatory response. LPS-induced cytokine release models have fast kinetics and model the acute phase of the cytokine storm.

Our catalogue or customized in vivo CRS models recapitulates key clinical and pathological features of CRS, forming an integrated platform for preclinical drug development. Creative Biolabs also offers in vitro CRS models. Please feel free to contact us to discuss your next study.

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