• CellRapeutics™ CAR Technology
• CellRapeutics™ TCR Technology
• CAR × TCR Bispecific T Cell Engineering Platform
• Surfarray™ Cell Microarray Technology
  • High-throughput Membrane Protein Array Technology
• T-Scan Platform for T Cell-Specific Epitope Identification
• Animal Model Platform for CAR-T Therapeutic Research
  • Pronuclear Injection-based HIS Mouse Model Platform
• Gamma Delta (γδ) T Cell Platform
• Tetravalent Bispecific (TetraBi) T Cell Engager Platform
• CAR-T Cell Display Platform
• CAR-Stem Cell Platform
• CRISPR-edited CAR Cell Technology
• Stable Packaging Cell Line (PCL) Platform
• Immune Monitoring Platform
• mRNA-based CAR Cell Platform
• Tseeking™ Platform For TCR/Ligand Discovery

Pronuclear Injection-based HIS Mouse Model Platform

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The Pronuclear Injection-based HIS (Humanized Immune System) Mouse Model Platform is a cutting-edge technology that has revolutionized the field of immunology and preclinical research. It involves the creation of humanized mouse models by introducing a functional human immune system into the mice. This is achieved through the pronuclear injection of human hematopoietic stem cells (HSCs) into mouse embryos, generating a chimeric model with a fully functional human immune system. Creative Biolabs offers this powerful tool to study human immune responses and diseases in a physiologically relevant in vivo setting.

Steps of Pronuclear Injection (PNI)-based Transgenic Mouse Model Generation

The key to the success of the HIS Mouse Model Platform lies in the precise and delicate techniques involved in generating these models. Creative Biolabs employs a cutting-edge technology that ensures the efficient engraftment and development of human immune cells within the mice. This technology includes:

1. Pronuclear Injection:
   Pronuclear injection is a microinjection technique used to deliver human HSCs into the pronuclei of mouse zygotes. This method enables the integration of human immune cells into the developing mouse embryos, resulting in the creation of humanized mice.
2. Selection and Validation:
   To ensure the quality of the models, our platform includes rigorous selection and validation processes. We use advanced screening methods to identify chimeric mice with a well-developed human immune system, enhancing the accuracy of research outcomes.
3. Immune System Characterization:
   Characterization of the immune system within the HIS mouse models is an essential aspect of our technology. We employ flow cytometry, gene expression analysis, and functional assays to assess the human immune system's functionality.

Fig.1 Transgenic mice by pronuclear injection of a transgene.¹

Features of the Pronuclear Injection-based HIS Mouse Model Platform

Creative Biolabs' Pronuclear Injection-based HIS Mouse Model Platform offers a host of features that set it apart from traditional models and other humanized mouse models:

1. Human Immune System in a Murine Host:
   This platform results in the creation of a mouse model with a human immune system, allowing for the study of human-specific immune responses in vivo.
2. Disease Modeling:
   These HIS mouse models provide a unique platform for the investigation of human immune system-related diseases, including cancer, infectious diseases, and autoimmune disorders.
3. Evaluation of Immunotherapies:
   Researchers can utilize our models to assess the efficacy and safety of immunotherapies, such as CAR-T cell therapy, in a preclinical setting.
4. Translational Research:
   The HIS mouse models facilitate the translation of research findings from bench to bedside, contributing to advancements in clinical medicine.

Reference

1. Oji, Asami, et al. "CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice." Scientific reports 6.1 (2016): 31666.