CD117

Product List

In addition to a range of anti-CD117 CAR-T services, Creative Biolabs also provides you with customized, high-quality CD117 CAR products. Please browse the CD117 CAR product list to find the product you are interested in.

Background

CD117, cluster of differentiation 117, is a gene encoding a receptor tyrosine kinase protein. C-kit is another familiar name. C-kit protein plays an important role in cell proliferation, differentiation, survival, and migration by activating various signaling pathways. It belongs to the type III receptor tyrosine kinase, and when bound to SCF, it undergoes dimerization, which in turn activates downstream signaling pathways by phosphorylation and activates signal transduction molecules, causing cell growth and differentiation. Studies have found that c-kit functional mutations are associated with diseases such as gastrointestinal stromal tumors (GIST), mantle cell lymphoma (MCL), and acute myeloid leukemia (AML).

Fig.1 Signaling pathways involved in CD117.¹

Associated Diseases

Chronic Lymphocytic Leukemia

Gastrointestinal Stromal Tumor

Mantle Cell Lymphoma

Acute Myeloid Leukemia

Anti-CD117 CAR-T Services

CD117 CAR Design & Construction

The modular design of the CAR consists of four main parts: the antigen-binding region, the hinge region, the transmembrane region, and the intracellular signal transduction region. Each component has a unique function, and Creative Biolabs achieves optimal molecular design for CAR through multiple variations of component proteins.

Fig.2 Flow diagram for generating CD117 CAR-T cells.²

Anti-CD117 CAR-T In Vitro Assays

Anti-CD117 CAR-T Expression Test

Creative Biolabs offers flow cytometry and western blot-based assays to help customers assess CAR expression levels on T cells or other cells.

Fig.3 CD117 CAR expression levels were determined using flow cytometry. The results showed high expression of CD117 CAR.²

Anti-CD117 CAR-T Cytokine Release Test

At Creative Biolabs, we offer different assays to help you assess the expression levels of CD117 CAR T cell secretory factor.

Fig.4 Secretion of pro-inflammatory cytokines in CD117 CAR T cells.²

Anti-CD117 CAR-T In Vitro Cytotoxicity Assay

Assessing the functional activity of CAR T cells in vitro is important for the overall immune cell therapy process. Creative Biolabs provides services for the cytotoxic activity of CD117 CAR. The figure below shows CD117 CAR T cells co-cultured with AML cells and then sorted to express luciferase, GFP, and human CD117.

Fig.5 Evaluation of the killing activity of CD117 CAR T cells. The results showed that CD117 CAR T cells exhibited potent cytotoxic activity.²

Anti-CD117 CAR-T In Vivo Assays

Anti-CD117 CAR-T Cell Therapy Animal Models

Creative Biolabs also provides in vivo activity assessment services for CD117 CAR T cells. We evaluated the efficacy and safety of CD117 CAR T cells in vivo by constructing a humanized mouse model and establishing the corresponding effector function.

Fig.6 Schematic of the anti-CD117 CAR-T cell therapy mouse model.²

Efficacy Test of Anti-CD117 CAR-T

Creative Biolabs provides in vivo efficacy services to evaluate the activity of CD117 CAR-T cells in different mouse models.

Fig.7 In vivo potency of CD117 CAR-T cells. The results showed that CD117 CAR-T mice had a better survival advantage compared to control mice.²

References

1. Foster, B.M.; et al. "CD117/c-kit in cancer stem cell-mediated progression and therapeutic resistance." Biomedicines. (2018) 6,1 31.
2. Magnani, C.F.; et al. "Anti-CD117 CAR T cells incorporating a safety switch eradicate human acute myeloid leukemia and hematopoietic stem cells." Molecular therapy oncolytics. (2023) 30 56-71.