CDH5 Membrane Protein Introduction

Introduction of CDH5

CDH5, the full name being cadherin-5, is also known as 7B4 antigen, vascular endothelial cadherin (VE-cadherin) or CD144. It belongs to the superfamily of classical cadherins and as such, mediates homotypic calcium-dependent cell-cell interactions. CDH5 expression is tissue-specific and unique to endothelial cells.

Basic Information of CDH5
Protein Name	Cadherin-5
Gene Name	CDH5
Aliases	7B4 antigen, Vascular endothelial cadherin (VE-cadherin), CD144
Organism	Homo sapiens (Human)
UniProt ID	P33151
Transmembrane Times	1
Length (aa)	784
Sequence	MQRLMMLLATSGACLGLLAVAAVAAAGANPAQRDTHSLLPTHRRQKRDWIWNQMHIDEEKNTSLPHHVGKIKSSVSRKNAKYLLKGEYVGKVFRVDAETGDVFAIERLDRENISEYHLTAVIVDKDTGENLETPSSFTIKVHDVNDNWPVFTHRLFNASVPESSAVGTSVISVTAVDADDPTVGDHASVMYQILKGKEYFAIDNSGRIITITKSLDREKQARYEIVVEARDAQGLRGDSGTATVLVTLQDINDNFPFFTQTKYTFVVPEDTRVGTSVGSLFVEDPDEPQNRMTKYSILRGDYQDAFTIETNPAHNEGIIKPMKPLDYEYIQQYSFIVEATDPTIDLRYMSPPAGNRAQVIINITDVDEPPIFQQPFYHFQLKENQKKPLIGTVLAMDPDAARHSIGYSIRRTSDKGQFFRVTKKGDIYNEKELDREVYPWYNLTVEAKELDSTGTPTGKESIVQVHIEVLDENDNAPEFAKPYQPKVCENAVHGQLVLQISAIDKDITPRNVKFKFILNTENNFTLTDNHDNTANITVKYGQFDREHTKVHFLPVVISDNGMPSRTGTSTLTVAVCKCNEQGEFTFCEDMAAQVGVSIQAVVAILLCILTITVITLLIFLRRRLRKQARAHGKSVPEIHEQLVTYDEEGGGEMDTTSYDVSVLNSVRRGGAKPPRPALDARPSLYAQVQKPPRHAPGAHGGPGEMAAMIEVKKDEADHDGDGPPYDTLHIYGYEGSESIAESLSSLGTDSSDSDVDYDFLNDWGPRFKMLAELYGSDPREELLY

Function of CDH5 Membrane Protein

CDH5 is a component of endothelial cell-cell adhesion junctions that plays a key role in maintaining vascular integrity. It is one of the first cell-specific markers expressed by endothelial cells during embryonic vasculature development. Early expression of CDH5 in embryonic endothelial progenitor cells suggests that it contributes to endothelial differentiation. In addition, CDH5 contributes to the critical and specific functional properties of endothelial cells during angiogenesis. These include the establishment of cell polarity, the formation of a correct lumen, vascular tubulogenesis, rearrangement of the cytoskeleton, tight junction remodeling and gene transcription.

CDH5 Membrane Protein Introduction Fig.1 CDH5-mediated endothelial cell adhesion. (Giannotta, 2013)

Application of CDH5 Membrane Protein in Literature

Liu J., et al. Chlamydia pneumoniae infection promotes monocyte transendothelial migration by increasing vascular endothelial cell permeability via the tyrosine phosphorylation of VE-cadherin. Biochem Biophys Res Commun. 2018, 497(2):742-48. PubMed ID: 29462613

Chlamydia pneumoniae infection promotes transendothelial migration of monocytes by increasing vascular endothelial cell permeability through tyrosine phosphorylation and VE-cadherin internalization in vascular endothelial cells.
Rezaei M., et al. The expression of VE-cadherin in breast cancer cells modulates cell dynamics as a function of tumor differentiation and promotes tumor-endothelial cell interactions. Histochem Cell Biol. 2018, 149(1):15-30. PubMed ID: 29143117

The study shows a VE-cadherin-mediated cell dynamics and an endothelial-dependent proliferation in a differentiation-dependent manner.
Inokuchi M., et al. Cadherin 5 is a significant risk factor for hematogenous recurrence and a prognostic factor in locally advanced gastric cancer. Anticancer Res. 2017, 37(12):6807-13. PubMed ID: 29187459

By analyzing the tumor tissues of patients with advanced gastric adenocarcinoma undergoing gastrectomy, authors find that CDH5 may play a key role in the recurrence of advanced gastric cancer and may be a viable therapeutic target.
Mikus M., et al. Elevated levels of circulating CDH5 and FABP1 in association with human drug-induced liver injury. Liver Int. 2017, 37(1):132-40. PubMed ID: 27224670

By using an affinity proteomics strategy, antibody suspension bead arrays are used to analyze plasma and serum samples from human DILI cases and controls. It is concluded that the expression levels of CDH5 and FABP1 are both elevated in drug-induced liver injury.
Bartolomé R.A., et al. VE-cadherin RGD motifs promote metastasis and constitute a potential therapeutic target in melanoma and breast cancers. Oncotarget. 2017, 8(1):215-227. PubMed ID: 27966446

The article supports the general role of VE-cadherin and other RGD cadherins as key regulators of lung and liver metastasis in multiple solid tumors.

CDH5 Preparation Options

Membrane protein studies have advanced significantly over the past few years. Based on our versatile Magic™ membrane protein production platform, we could offer a series of membrane protein preparation services for worldwide customers in reconstitution forms or membrane protein analysis. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-CDH5 antibody development services.

During the past years, Creative Biolabs has successfully generated many functional membrane proteins for our global customers. We are happy to accelerate the development of our clients’ programs with our one-stop, custom-oriented service. For more detailed information, please feel free to contact us.

Reference

Giannotta M, et al. (2013). VE-cadherin and endothelial adherens junctions: active guardians of vascular integrity. Dev Cell. 26(5), 441-54.

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