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Mammalian Cell Lysate based Cell-free Membrane Protein Expression Service

Magic™ Mammalian Cell-Free Membrane Protein Expression is a cell-free protein synthesis system using human cell extracts to rapidly express and manufacture large amounts of target proteins. This system contains all the required components for protein synthesis (ribosomes, translation initiation/elongation/enhancement factors, tRNA, polymerase, amino acids, ATP, etc.) and allows membrane protein synthesis to occur at high efficiency and high yield. Modifications such as glycosylation and phosphorylation could be easily obtained. The procedure can be performed in a single reaction tube from RNA transcription to protein synthesis. This in vitro cell-free expression system provides the benefits of microliter scale reactions and speed. With results available in a few hours, the kit saves valuable laboratory time and is ideal for high throughput technologies.

Cloning Vector

The full length of the cloning vector is 3355 bp (see Fig.1). For proper expression, all templates must contain the T7 promoter, an initiation codon, and an EMCV internal ribosome entry site (IRES) to facilitate high levels of in vitro membrane protein expression.

Magic™ Cell-free Membrane Protein Expression in Mammalian Cell LysateFig.1 Cloning vector.

Components List and Storage Information

Expression System Components Storage
In Vitro Mammalian Cell Expression System Human Cell Extract -80°C
Reaction Buffer -80°C
Accessory Proteins -80°C
Amino Acid Mix -80°C
Cloning Vector -80°C
T7 RNA Polymerase -80°C
Control Vector -80°C

Features

  • Synthesized in as little as 1 hour and easy setup with all necessary components in a single-tube reaction
  • Provides high yield and functional protein
  • Enables the synthesis of large proteins (such as over 150 kDa)
  • Modifications such as glycosylation and phosphorylation could be easily obtained
  • Translation enhancement factor included in the accessory proteins ensures the high translation level
  • Various applications: high throughput screening, synthetic biology, toxic or difficult to express protein synthesis, studies on protein folding, activity, and protein-protein interactions

Applications

  • Expression of toxic proteins
  • Quick analysis of protein functionality, mutations, or truncations
  • High-throughput proteomic studies

Creative Biolabs is a world-leading service provider of cell-free membrane protein production. Moreover, we now provide integrated Magic™ cell-free membrane protein expression system in mammalian cell lysate for our clients around the world. To get more information, please feel free to contact us.

Related Products

Cat Product Name Expected Yield of Positive Control
MPCFK-2 Magic™ Cell-free Membrane Protein Expression in Mammalian Cell Lysate 0.75 mg/mL
MPCFK-2-2 Magic™ Cell-free Membrane Protein Expression in Human Hela Cell Lysate 0.1 mg/mL

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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