Creative Biolabs has perfect qualifications and strong technical capabilities, we are committed to providing high-quality guaranteed service and technical support for scientific researchers all over the world. With their rich experience in biological experiments, our research team will provide you with the most comprehensive and robust complement-related experimental solutions.
SP-D Purification
The surface-active protein SP-D, known as collectin, is a multifunctional, pattern-recognizing innate immune molecule. It is an important component of the body's defense system, participates in pathogen recognition and regulation of lung inflammatory response, and plays an inhibitory role in the pathological process of several lung infections. SP-D is predominantly present in type II pneumocytes, with the highest expression observed in alveoli. SP-D can also be detected and collected in extrapulmonary tissues, such as plasma, serum or amniotic fluid. Here, we briefly describe the protocol for purifying SP-D from amniotic fluid, which will serve as the basis for any SP-D-related research and provide a guarantee for the success of subsequent analysis experiments. This protocol is only suitable for the purification of SP-D in amniotic fluid samples, but can also be extended to other samples after simple modification.
SP-D Purification Protocol
Fig 1. Workflow of the purification of SP-D in amniotic fluid samples. (Creative Biolabs)
Good results require the support of excellent, high-purity samples. As a technical expert who has been focusing on complement research for many years, Creative Biolabs has always committed to providing the highest quality experimental tools and solutions for researchers. You may directly purchase our certified and well-characterized reagents, or obtain a series of cell lines or biological products quickly and customized. The first-class technology platform and experimental deployment capabilities allow us to have sufficient confidence to complete any biologically related detection, analysis or development experiment you entrust. Please do not hesitate to contact us and consider that your project has been greatly advanced.
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Please note that our protocols are only for your reference!
For Research Use Only.
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