As one of the important media of intercellular communication, extracellular vesicles (EVs) have the characteristics of high stability, strong ability to enter tissue cells, and good biocompatibility, and have inherent advantages in disease treatment. However, native EVs are poorly targeted and easily cleared from circulation. A large number of studies have shown that engineering transformation is expected to improve the targeting and anti-phagocytosis capabilities of EV and enhance the internalization of target cells. EV engineering strategies mainly include two aspects: EV drug loading and EV surface modification. Engineered EV can perform a variety of functions, including targeted delivery of drugs, regulation of specific gene expression, promotion of immune responses, and combination of multiple therapeutic methods, etc., and have broad clinical application prospects. Creative Biolabs has been paying attention to the research progress of engineered EVs, and has been continuously improving technology and equipment, and is willing to work with customers to develop the therapeutic potential of EV.

Fig.1 Current technologies for EV bioengineering.^1,2

Therapeutic Molecular Loading Services for Extracellular Vesicle

We can provide EV loading service to load nucleic acids (including mRNA, miRNA, circRNA, siRNA, etc.), proteins, and drugs into EV. The loading techniques we use include genetic loading and physicochemical loading. Genetic loading refers to the overexpression of therapeutic small molecules in parental cells, relying on the cell's own sorting system to complete the loading. Physicochemical loading refers to the loading of therapeutic small molecules into EVs by physical or chemical means after the isolation and purification of EV.

Surface Modification Services for Extracellular Vesicle

The EV surface modification services we can provide include targeting service (endowing EV tissue or cell targeting, anti-phagocytosis, and internalization capabilities) and labeling service (in vivo or in vitro tracking). The modification techniques we use are divided into the genetic modification and chemical modification. Genetic modification refers to the genetic recombination of parental cells to produce EV with modified peptides or proteins. Chemical modification refers to the modification of functional molecules on the surface of EV by chemical methods after the isolation and purification of EV.

Creative Biolabs has been focusing on the development of cell-free therapy. In addition to EV modification services, we can provide global customers with EV one-stop services including isolation and purification services for EV, characterization and quantification services for EV, EV profiling services, EV manufacturing service, and EV functional research and verification services. If you want to get engineered EV with more comprehensive functions, please contact us. Our professional team will formulate the most rigorous experimental plan for you.

References

1. Weng, Z.; et al. Therapeutic roles of mesenchymal stem cell-derived extracellular vesicles in cancer. Journal of Hematology & Oncology. 2021, 14(1):136.
2. under Open Access license CC BY 4.0, without modification.

• Isolation & Purification
• Characterization & Quantification
• Extracellular Vesicle Profiling Services
• Extracellular Vesicle Manufacturing Service
• Functional Research and Verification Services of Extracellular Vesicle