Single-cell transcriptomics has been widely used in many fields such as tumor immunity, tumor microenvironment, neurodevelopment, reproduction, etc., and plays an important role in analyzing the tissue microenvironment and solving the problem of cell heterogeneity. But single-cell transcriptomics has reached a bottleneck. Tissue exosomes are exosomes that exist in the interstitial space of tissue cells. Due to the heterogeneity of exosomes secreted by different cells in a specific tissue, researchers have gradually focused on the joint study of exosomes and single-cell transcriptomics to find the parent cells of different exosome subsets. This can provide a basis for the study of the mechanism of exosome-based intercellular communication and intertissue communication. Creative Biolabs can provide efficient tissue exosome extraction service and single-cell transcriptomic analysis to help customers clarify the exosome communication network.

Single-Cell and Tissue Exosome Combined Transcriptomic Research Application

Finding the traceability of key molecules in tissue exosomes can provide an important basis for solving the action mechanism of exosomes and the target of disease treatment. A research team found that THBS2 protein was associated with lung cancer malignancy through data mining of public databases and found that THBS2 was highly enriched in lung cancer tissue exosomes. To find out which cells THBS2 in these exosomes originated from, they performed single-cell transcriptomics of lung cancer tissues and further found that the THBS2-expressing cell subsets were tumor-associated fibroblasts (CAFs). Subsequently, through in-depth analysis and functional studies, it was found that in early invasive lung adenocarcinoma, CAFs secreted THBS2 through exosomes to regulate the tumor microenvironment, affecting tumor immunity, recurrence, and treatment resistance.

Fig.1 The workflow showing the evaluation of responses to immune checkpoint inhibitors (ICIs) in LUAD patients.^1,2

Single-Cell and Tissue Exosome Combined Transcriptomic Research Process

At Creative Biolabs, for the same tissue sample provided by customers, we can provide efficient single-cell dispersion service and tissue exosome extraction service through differential centrifugation, ultracentrifugation, molecular size exclusion, and ultrafiltration. After the identification of tissue exosomes, we can analyze the tissue microenvironment from the two dimensions of cell subsets and intercellular exosomes through high-throughput transcriptomic analysis. In addition, we can also help customers fully reveal the exosome communication network between cell subpopulations in the tissue microenvironment and discover disease-related biomarkers and drug targets by providing follow-up tissue exosome content analysis service and tissue exosome functional research service.

Exosome-based liquid biopsy and drug delivery research are getting more and more in-depth, and many studies have entered different clinical stages. However, there are still key issues to be solved in the field of exosome research, such as the traceability of exosomes and the function of exosome subsets. As a benchmark enterprise engaged in exosome research and scientific research services for many years, Creative Biolabs always pays attention to the latest developments in the exosome field and thinks about how to improve technology and strategies to provide customers with more innovative and advanced technical services and exosome research solution. If you also want to trace the origin of exosome subsets and study their functions, please contact us without hesitation and leave your specific needs. Based on our most comprehensive omics platform, our professional team can provide evidence for your scientific questions through the most rigorous bioinformatics results.

References

1. Yang, H.; et al. Multi-scale integrative analyses identify THBS2+ cancer-associated fibroblasts as a key orchestrator promoting aggressiveness in early-stage lung adenocarcinoma. Theranostics. 2022, 12(7):3104-3130.
2. under Open Access license CC BY 4.0, without modification.

• Body Fluid Exosome & Tissue Exosome Combined Research
• Single-Cell & Tissue Exosome Combined Proteomics Research
• Tissue Exosome Related Functional Research and Verification
• Tissue Exosome Related Therapeutic Research