Large-scale production of exosomes can meet the needs of academia and industry for exosome products, laying a solid foundation for transforming exosome research into applications. In the large-scale production process, the stability evaluation of exosome products is indispensable. In order to meet the needs of customers, Creative Biolabs has specially deployed the stability evaluation business of exosome products.
There are differences in the stability of exosomes under different preparation methods and storage conditions. Improper handling may damage and even degrade exosomes, resulting in changes in their composition, activity, and function, thereby affecting their application effects. Therefore, the evaluation of exosome product stability helps in the following ways.
Using rich experience and advanced technology platforms, Creative Biolabs will provide the best exosome product stability evaluation service.
STABILITY EVALUATION ANGLE | EVALUATION REASON | TEST METHODS | EVALUATION METHOD |
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Preparation method | Although some preparation methods ensure the purity of exosomes, they have a certain degree of damage to exosomes. Currently, commonly used exosome isolation methods include ultracentrifugation, ultrafiltration, affinity chromatography, size-exclusion chromatography, and Precipitation. The quality of exosomes obtained by these methods is also different. | Quality comparison of exosomes from different preparation methods |
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Storage temperature | Since frequent freezing/thawing processes may cause damage to exosomes during cryopreservation, it is necessary to evaluate the stability of exosomes after 1-2 freezing/thawing cycles. | Freeze/thaw test | |
In general, exosome products can be cryogenic or freezing. Therefore, it is necessary to evaluate the stability of cryogenic or frozen exosomes. | Storage temperature comparison | ||
Storage time | As biologically active vesicles, exosomes may be degraded and lose their effectiveness after being placed for a long time. Therefore, it is necessary to compare the differences in parameters between fresh exosomes and exosome products after a period of storage. | Comparison at different storage time points | |
Stock buffer composition | Adding some protective agents to the storage buffer can improve the stability of exosomes. Therefore, it is necessary to compare the protective effects of different buffer formulations on exosomes. | Stock buffer composition comparison | |
Other feasible evaluation angles | Concrete analysis of specific situations |
Fig.1 Exosome evaluation by nanoparticle size analysis, transmission electron microscopy and CD81 identification.
Through these refined analyses and evaluations, we will ensure that the quality and stability of exosome products are maximized, and help customers ensure the reliability of exosome products. If you need to evaluate the stability of your exosome product, please contact us and tell us the specific information about the product and testing requirements to get a complete quotation.
A: Storage temperature and duration are critical factors affecting the stability of exosomes. We conduct systematic tests to determine the optimal storage conditions, ensuring that exosomes maintain their activity and efficacy during storage and transport.
A: Clients need to provide detailed information about the source of the exosomes, their intended application, and the preparation method. This information helps us to develop a targeted stability assessment plan.
A: We provide detailed data analysis and reports, including all results from the stability tests. Additionally, we offer expert improvement recommendations based on the assessment results to help clients optimize the stability of their exosome products.