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FACS-Positive Antibody Discovery Service

Overview FACS Antibodies High-throughput FACS Significance Key Features

Overview of Fluorescence-Activated Cell Sorting (FACS)

FACS is a particular type of flow cytometry that highly relies on antibodies labeled with fluorescent conjugates. In addition to simultaneously collecting multiple data on biological samples, FACS can also classify biological samples by different parameters. Importantly, FACS is capable of rapidly recording the fluorescence signals of single cells, as well as effectively isolating desired cells. Thus, FACS has proved to be a useful tool for studying the cellular basis of health and disease. Currently, FACS also plays a crucial role in FACS-positive hybridoma screening.

FACS Antibodies

FACS antibodies are key media for flow cytometry detection. In general, fluorescently conjugated antibodies are suitable for direct detection while unconjugated antibodies are suitable for indirect detection. The quality of the antibody is decisive for flow cytometry detection. Antibodies used in FACS play a crucial role in detecting low-abundance markers. Furthermore, FACS contributes to determining the efficiency of specific treatments, which greatly facilitates the development of targeted or personalized treatments.

Fig.1 Outline of FACS procedures. (He, et al., 2015)Fig.1 Procedures for FACS analysis of heterotypic CICs.1

High-throughput FACS Technology

Although screening available hybridomas is a complex and arduous task, the emergence of various high-throughput technologies has provided new opportunities for screening target hybridomas. FACS technology represents an attractive high-throughput technology for in vitro screening of positive hybridomas. Creative has developed an advanced High-throughput FACS Hybridoma Screening Platform. Within this platform, we can screen hundreds of candidate clones with high speed and high sensitivity. To obtain FACS-positive hybridoma clones, usually we perform the high-throughput FACS screening from the specific screening of clones, and specific screening of sub-clones, to the final verification of antibodies. Our experts with years of screening experience will give you the best screening solution to promote your project to success.

Fig.2 High-throughput FACS Technology. (Creative Biolabs Original)Fig.2 Specific screening of hybridoma clones by FACS. (Creative Biolabs Original)

Why Perform FACS Verification?

Features of Our Services

Our team is an expert in hybridoma screening, resulting in a final product that is highly specific for your FACS experiments.

Our validated FACS hybridoma screening strategy significantly improves the feasibility of antibodies for FACS applications.

We can complete FACS-positive hybridoma screening in a shorter time with a complete experimental report.

Creative Biolabs has been committed to providing suitable hybridoma development services to customers in more than 30 countries and regions around the world. Based on our rich experience and expertise in hybridoma antibody production, we are willing to provide you with a cost-effective FACS-positive hybridoma screening service. If you are looking for custom antibodies for FACS use, please contact us for more details about customization services.

Reference
  1. He, Meifang, et al. "Fluorescence-activated cell sorting analysis of heterotypic cell-in-cell structures." Scientific Reports 5.1 (2015): 9588.
    Distributed under Open Access license CC BY 4.0, without modification.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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