Functional Guaranteed Antibody Discovery Services

Introduction Workflow Hybridoma Screening Techniques

Antibodies are indispensable instruments in research owing to their exceptional specificity, strong binding affinity, extended lifespans, and minimal toxicity. At Creative Biolabs, our top-tier hybridoma development services cater to a wide array of assay requirements including ELISA, western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), fluorescence-activated cell sorting (FACS), and beyond. We offer customized and adaptable solutions tailored precisely to meet your unique needs.

Introduction of Function-Guaranteed Antibody Development

Fig. 1 Common applications of antibodies. (Creative Biolabs Original)

For efficient interaction between an antigen and antibody, it is essential that the epitope remains readily accessible for binding. Any alterations to the target molecule, such as denaturation due to fluctuations in pH, fixation, reduction, or during the preparation process, can potentially modify the epitope, consequently impacting its ability to engage with an antibody. Occasionally, antibodies may prove ineffective in WB assays but demonstrate suitability for IHC applications. In IHC assays, a complex antigenic site may persist within the tissue; however, in WB assays, the proteins undergo denaturation under conditions that sufficiently alter their conformation, thereby disrupting the antigenic site and subsequently preventing antibody binding. Consequently, antibodies generated against native proteins are more likely to exhibit optimal reactivity with native proteins (e.g., in immunoprecipitation (IP) or FACS), while antibodies raised against denatured proteins exhibit reactivity with proteins that have been subjected to denaturing conditions (e.g., in WB).

Workflow of Function-guaranteed Antibody Development

Fig.2 Workflow. (Creative Biolabs Original)

Hybridoma Screening

Subsequent to fusion and selection with HAT medium, hybridomas undergo screening using various techniques to identify the presence of antibodies targeting the antigen. Initially, we ascertain the subclass and titer of the antibodies. Cells originating from wells showing positive results are expanded and cryopreserved to provide ample time for validation of the initial supernatant. The primary supernatants from hybridoma cells exhibiting positivity are subsequently subjected to validation in specified assays including WB, IP, IF, FACS, and IHC.

Guarantee	At every decision point, we provide assurances tied to results. You retain oversight of the process while delegating risks to us.
Efficiency	From target selection to final validation, we guarantee service quality and a short turnaround time.
Freedom to Operate	You possess complete control over, and sole ownership of, all generated antibodies.
Enhanced Success Probability	We possess strong technical capability as the backing.
Wide Range of Solutions	We offer a complete toolbox to deliver you a candidate with optimal properties.

Choosing the Appropriate Hybridoma Screening Technique

Antibodies in research identify proteins intracellularly and extracellularly. We differentiate cell types in FACS, detect proteins in WB, and isolate proteins in IP. Also, we analyze protein expression in tissue sections or localize proteins within cells in IHC or IF. ELISA quantifies proteins using antibodies. Considering varied applications, it's crucial to align hybridoma screening methods with intended uses. Creative Biolabs offers function-guaranteed hybridoma development services tailored to diverse research needs.

With a wealth of expertise in mAb production, Creative Biolabs is acknowledged as a frontrunner in this domain. Bolstered by our cutting-edge hybridoma facilities, we ensure the provision of a wide array of hybridoma-related services. We eagerly await your to contact us for additional information.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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