AAV Vector Design for Basic Research
Viral vectors are the most commonly used DNA delivery vectors for gene therapy. Among them, adeno-associated virus (AAV), a class of small viruses, provides long-term transgene expression with high efficiency and safety. Creative Biolabs offers a comprehensive range of AAV viral vectors and advanced viral vector design techniques for basic research such as gene expression, gene editing, gene addition, and gene replacement.
AAV Vector Design for Basic Research
Based on the integrated delivery systems development platform, Creative Biolabs is dedicated to designing a wide variety of AAV vectors for basic research applications. Our basic vector design concepts encompass protein-coding gene expression and small non-coding RNA gene expression cassettes, AAV plasmid vector backbone modifications, and key sequence mutations of various modular expression components, which can be inserted as needed for the specific application. Specifically, our AAV vector can be designed for the following applications:
Figure 1. AAV-based Gene Therapy.
Our creative viral vector design approach allows for gene overexpression, or conditional expression. Our platform demonstrates the reproducibility and reliability of AAV viral vectors and is well suited for target gene identification and in vivo validation.
Viral vectors can also serve as important vectors for gene editing. Unlike random insertion of early genetic engineering techniques, AAV can insert genetic material into a specific location in the host genome by carrying the target gene of interest (<4 kb inserts).
AAV vector-mediated gene addition can be achieved by different mechanisms, such as by chromosomal integration or episomal transgene expression. We have used AAV to introduce a mutant alkaline phosphatase reporter gene into normal human cells, which are then calibrated with the AAV gene targeting vector.
AAV-based gene replacement can be used not only for basic research, but also for the potential of translational therapy in clinical applications of gene therapy. We have used a combination of restriction enzymes to replace modular expression cassette components, which can be assembled into an AAV vector.
Highlighted Features
- Cost-effectiveness
- Customized solutions
- Executed by qualified engineers
- Extremely effective
Creative Biolabs focuses on designing AAV vectors to alter tropism, improve delivery efficiency and evade antibody neutralization. We offer novel molecular engineering and directed evolution methods for AAV vectors to generate enhanced AAV gene delivery vectors to meet the needs of basic research. Please feel free to contact us for more details and we are happy to provide you with the best service.
