AAV Vector Design for Gene Expression
Creative Biolabs is pleased to share our cutting-edge technology and extensive expertise in the field of viral vector development with our customers. We offer high-quality customized services to develop well-validated and highly published AAV vectors with your gene-of-interest. We are building a team of talented and motivated scientists and technicians to pursue our commitment and have won a good reputation among our worldwide customers for successfully accomplishing numerous challenging projects in this field.
AAV Vector Design for Gene Expression
Gene therapy has been considered as a novel approach that holds great promise for a wide variety of disease therapy in humans. As one of the most effective gene transfer systems, many properties of AAV vectors have been proven in previous studies, such as no or low level of cytotoxicity, the absence of host inflammatory, as well as the broad tropism. Meanwhile, scientists have revealed that AAV vectors can be widely used for mediating stable and long-term gene expression in different kinds of tissues. Moreover, further researches on improving the titer and purity of AAV vectors will accelerate the step of in vivo studies and provide the data needed for gene therapy. In the AAV system, a number of help viruses, including adenovirus and herpes simplex virus (HSV), have also been identified. The results suggest that these viruses play an important role in providing proteins that are necessary for AAV replication. In addition, the gene expression of AAV vectors in the brain have been analyzed by injecting the caudate nucleus with AAV in animals. The data derived from X-gal histochemistry indicates that the AAV vector is a safe and effective method to change in vivo gene expression in neurons.
Figure 1. AAV Vector Design Services for Gene Expression.
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The AAV vector system is a popular and powerful tool for in vitro and in vivo gene delivery. Pilot studies have shown that AAV can effectively transduce a collection of cell types with low immunogenicity or cytotoxicity in various animal models. In general, AAV vectors are designed by inverting target genes into or between the two inverted terminal repeats (ITRs). AAV vectors are added to infect specific cells and the single-stranded linear DNA genomes are converted into double-stranded DNA genomes.
Currently, Creative Biolabs provides a series of AAV vector design solutions for our worldwide customers to improve the gene expression in animal studies. For instance, we have successfully evaluated the long-term gene expression of AAV vectors in specific sites of the rat brain. Additionally, many stains of AAV have been confirmed by our labs, and many serotypes of AAV can be used for designing a number of AAV vectors with different tissue infection specificity. Equipped with advanced technology, we are always dedicated to offering a battery of safe, low risk, high gene expression AAV vectors for your projects. Nowadays, we have classified our AAV vector-based gene expression services into three groups basing on the gene types, including but not limited to:
As a world-leading service provider in AAV vector services, Creative Biolabs is passionate about offering valuable solutions to develop promising AAV vector-based gene expression services for our clients. Our mission is to help you meet current and future viral vector demands and clear the path to commercialization to help you stay at the forefront of AAV vector design and gene therapy. If you are interested in our services, please contact us for more details.
