Self-complementary AAV Vector Service

Recombinant adeno-associated virus (rAAV) has been successful in clinical trials for a variety of diseases. The self-complementary AAV (scAAV) is a recombinant viral vector engineered from a natural AAV and used as a tool for gene therapy. Creative Biolabs offers a comprehensive range of lab-made scAAV viruses with advantages including increased and extended transgene expression in vitro and in vivo, as well as higher in vivo DNA stability and more efficient cyclization.

Advantages of scAAV Vectors

Traditional rAAV gene delivery vectors package single-stranded DNA (ssDNA) and must rely on cellular replication factors to synthesize complementary strands. However, by packaging the two strands into a single (self-complementary) DNA molecule, our scAAV vector has a 10 to 100-fold increase in transduction efficiency over conventional rAAV. More importantly, unlike conventional single-chain AAV vectors, inhibitors of DNA replication do not affect the transduction of the scAAV vector. Furthermore, the scAAV vector has shown rapid onset and higher levels of transgene expression in our tests to date and has shown efficient transduction in vivo.

Schematic structure of conventional single-stranded (ss) and double-stranded (ds) self-complementary AAV vectors Figure 1. Schematic structure of conventional single-stranded (ss) and double-stranded (ds) self-complementary AAV vectors.

Applications of scAAV Vectors

Numerous studies have demonstrated that scAAV has a higher transduction efficiency in animal models compared to conventional rAAV. Although the size of scAAV vectors is limited, recent observations of larger genomes packaged in scAAV have greatly expanded the exciting potential of these vectors. The potential application of scAAV will depend on the ability to package genetically modified and related regulatory elements, which is the direction our scientists focus on.

  • Gene delivery with higher transduction efficiency in dividing and non-dividing cells
  • Generating research animal models
  • Gene therapy research
  • Persistent and non-integrated expression in non-dividing cells

Highlighted Features

  • One-stop service: DNA synthesis, plasmid construction and preparation, scAAV packaging and purification
  • High titer for animal injection
  • Multiple AAV serotypes for transduction of various cell types
  • Cost-effectiveness
  • Customized solution
  • Extremely effective

As a leading expert in viral technology, Creative Biolabs focuses on producing high titers and large-scale scAAV particles to meet your scientific needs. From initial sequence synthesis, viral plasmid construction to final viral production, Creative Biolabs can help you accelerate your research in every time-consuming step.

Please feel free to contact us for more details and we are happy to provide you with the best service.

Reference

  1. Srivastava, A. (2006). Adeno-associated Viral Vectors in Gene Therapy. eLS.
For research use only. Not intended for any clinical use.