Liver-targeting Adenovirus Vector Construction Service
Creative Biolabs has always been committed to providing customers with professional adenoviral vector construction services. Our scientists will work with you and keep in mind the safety, efficiency, purpose of optimizing services according to your requirements. Our adenoviral vector construction platform is perfect to construct different targeted adenoviral vectors, and liver-targeted adenoviral vectors represent the leading level of current technology. No matter what difficulties you have encountered in the relevant research work, you can contact us to get your customized service.
Liver-targeting gene therapy
The liver plays an important role in the development of many hereditary and acquired genetic diseases and is also a drug target for the treatment of some innate metabolic diseases. However, due to the important role of the liver in the metabolism of the human body, the traditional methods of treating liver-related diseases are limited by some side effects. The emergence of gene therapy technology has provided new ideas for the treatment of liver diseases - radically cure diseases by transferring specific genes to liver cells via some vectors.
The specificity is the difficult point that liver-targeting gene therapy (LTGL) needs to solve. To selectively remove cancerous cells in treatment without affecting normal cells, it is necessary to prevent toxic exchange between tumor cells and nearby healthy cells. In vitro gene therapy for human induced pluripotent stem cells (IPSCs) or autologous stem cells provides a promising approach to the healing of liver-related diseases. It is also possible to properly optimize and alter the gene for therapeutic delivery by targeting gene modification. In the future, once the relevant safety issues are resolved, such genetically modified stem cells can become an autologous transplant option for liver disease treatment. Although adenoviral vectors have limited efficacy in gene therapy for most liver diseases, they have shown the ability to efficiently transmit genes, which has attracted more and more scientists' attention.
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Adenovirus Vector for Gene Therapy
Some of the characteristics of adenovirus make them very suitable for gene therapy. First, their existence is very common. More than 50 serotypes of adenovirus have been reported in humans. Second, adenovirus has high efficiency in infecting human cells. It can quickly express the encoded protein of the target gene to a higher level. Third, the adenovirus has higher biosafety and less threat to humans, with only some mild infection symptoms. Fourth, the adenovirus vector can accommodate larger DNA fragments with high fidelity and easy handling. At present, in the treatment of liver cancer, an oncolytic adenovirus Ad-199T has been developed which has the ability to replicate under certain conditions, and the vector introduces four copies of the miR-199 target site in the 3'UTR of the E1A gene. The expression is strictly regulated by the levels of RNA and protein in primary hepatocellular carcinoma, which can rapidly remove liver cancer cells in mice without causing significant hepatotoxicity.
Creative Biolabs has sophisticated genetic construction techniques to build and optimize liver-directed adenoviral vectors. We can not only design a universal carrier with good applicability, but also customize additional optimization services for the genes you need to deliver. Providing you with a safe, stable and efficient service makes us responsible. If you have any question or have any difficulty, you can contact us by email or send us an inquiry to find a complete solution.
