Glial Cells-targeting Adenovirus Vector Construction Service
The nervous system is comprised of neurons supported and nourished by glial cells, and 90% of primary brain tumor cells are of glial origin. Glioma is the most common primary human brain tumor, and there is no effective treatment. Gene therapy has received attention as a promising treatment modality for malignant brain tumors. Creative Biolabs is the world's leading biotechnology company and has developed an excellent adenovirus vector construction platform for gene therapy. We offer a variety of glial cells-targeting adenovirus vectors to meet the needs of each customer.
Introduction of Glial Cells-targeting Adenovirus Vector
Glial fibrillary acidic protein (GFAP) is an intermediate filament that is expressed only in cells of glial origin. Therefore, the GFAP promoter is glial specific but weak. Most glioblastoma tumors do not express GFAP protein, and reporter gene studies indicated that the inhibition of GFAP promoter activity is mediated by methylation, and transient transfection of the GFAP promoter is still active in these cells. Studies have shown that an adenovirus construct containing multimers of a GFAP enhancer element coupled to a tetracycline transactivator (tTA) enables robust, repressible glial-specific ectopic gene expression. It was necessary to generate two adenovirus vectors. One virus contained tTA under the control a GFAP enhancer, and a second one contained the therapeutic or reporter gene regulated by tetracycline operator. Coinfection with both viruses enabled robust, tetracycline-repressible expression of β-galactosidase in human glioma cell lines but not in cells of nonglial origin. These adenovirus vectors are useful in a variety of gene therapy approaches for the central nervous system. Astrocyte-specific expression will be potentially applicable for replacement therapy. Alternatively, these viruses can also be used for cytotoxic or antiproliferative gene expression that would selectively target glioma cells and spare neurons and cerebral capillary endothelia.
Figure 1. Immunostaining (RED) of GFAP.
The Service of Glial Cells-targeting Adenovirus Vector Construction
Progress in gene therapy has allowed the development of several strategies against human brain tumors. Adenoviral vectors are excellent vehicles to transfer genes into the nervous system. Most are focused on the construction of improved vectors to enhance gene delivery, and, on the other hand, on the development of new therapeutic tools based on the restoration or destruction of a deregulated gene, the use of suicide genes, genetic immunopotentiation. Based on high-end scientists and professional platforms, we provide the above-mentioned adenoviral vector construction services and therapeutic tools.
Figure 2. The construction of the recombinant adenovirus vector.
Creative Biolabs has devoted to the development of gene therapies for years. We whole-heartedly cooperate with you to promote the successful completion of the projects. We have accumulated scientific experience from the accomplishment projects and are very proud of our high-quality platforms to meet diverse needs from our clients. If you are interested in our services, please contact us for more details.
