HSPC:Chol Liposomes (CAT#: LDLY-0123-LY9)

Rottlerin decreased the syncytial formation of FCoV.

This work investigates the rottlerin-liposome (RL)-induced inhibition of feline coronavirus (FCoV) infection. In order to obtain RL, researchers encapsulated rottlerin (R) in HSPC:Chol (55:45) liposomes. They compared R and RL's impact on the FCoV growth pattern in CRFK cells after that. The fluorescence intensity of the R and RL groups was much lower than that of the mock or liposome groups, as seen in stained cells under a fluorescence microscope (figure A-B). This indicates that both R and RL may inhibit FCoV replication. Moreover, the formation of syncytium is an indication of cell-cell fusion. The results (figure C) demonstrated a substantial reduction in the average number of nuclei per syncytium in RL and R treated cells compared to mock and liposome cells. These findings suggest that RL and R may inhibit FCoV syncytia formation at a later stage of infection, with RL exhibiting a greater effect. This work highlights that encapsulating R in HSPC:Chol (55:45) liposomes improve its inhibitory impact on FCoV, presenting a promising treatment option for FCoV infections.

Pubmed: 37368766 DOI: 10.3390/vetsci10060380

Choi, Jong-Chul, et al. "Rottlerin-Liposome Inhibits the Endocytosis of Feline Coronavirus Infection." Veterinary Sciences 10.6 (2023): 380.