Cholesterol is crucial for stabilizing the lipid bilayers in these liposomes, enhancing the structural integrity and permeability, which is vital for drug delivery applications.
Yes, these liposomes are often used for loading and delivering both hydrophilic and lipophilic drugs, improving the bioavailability and efficacy of the drugs.
They are primarily used in drug delivery systems, particularly for targeting cancer cells, due to their ability to encapsulate and efficiently deliver therapeutic agents.
These liposomes can be prepared using methods such as thin-film hydration followed by extrusion, which helps achieve uniform size and encapsulation efficiency.
The specific ratio of HSPC to cholesterol in these liposomes provides a balance between fluidity and rigidity, offering enhanced circulation time and stability in biological systems.
Rottlerin decreased the syncytial formation of FCoV.
This work investigates the rottlerin-liposome (RL)-induced inhibition of feline coronavirus (FCoV) infection. In order to obtain RL, researchers encapsulated rottlerin (R) in HSPC:Chol (55:45) liposomes. They compared R and RL's impact on the FCoV growth pattern in CRFK cells after that. The fluorescence intensity of the R and RL groups was much lower than that of the mock or liposome groups, as seen in stained cells under a fluorescence microscope (figure A-B). This indicates that both R and RL may inhibit FCoV replication. Moreover, the formation of syncytium is an indication of cell-cell fusion. The results (figure C) demonstrated a substantial reduction in the average number of nuclei per syncytium in RL and R treated cells compared to mock and liposome cells. These findings suggest that RL and R may inhibit FCoV syncytia formation at a later stage of infection, with RL exhibiting a greater effect. This work highlights that encapsulating R in HSPC:Chol (55:45) liposomes improve its inhibitory impact on FCoV, presenting a promising treatment option for FCoV infections.
Pubmed: 37368766 DOI: 10.3390/vetsci10060380
Choi, Jong-Chul, et al. "Rottlerin-Liposome Inhibits the Endocytosis of Feline Coronavirus Infection." Veterinary Sciences 10.6 (2023): 380.
Click the button below to contact us or submit your feedback about this product.
Online Inquiry