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Antigen Microarray Detection Service for Common Allergens

With several years of experience and expertise, Creative Biolabs has developed many types of antigen microarrays that can track a wide range of proteins, allowing for diagnostic and therapeutic studies on a variety of diseases. Our antigen microarray detection services for common allergens can help you diagnose multiple allergens to suit different allergy research and diagnostic applications.

Background

Overview of Allergens Diagnosis

Before the discovery of immunoglobulin E (IgE) as the reaginic substance in 1967, skin testing was the primary method for determining allergen sensitization. Since that time, serum-based in vitro tests have become an important tool for identifying specific IgE, a hallmark of allergic disease. IgE testing is now conducted routinely in clinical laboratories with high sensitivity and specificity. Over the past 2 decades, the emergence of molecular allergology has led to further advances in the field of IgE diagnostics. Historically, in vitro IgE testing was limited to crude allergen extracts, which represent a complex mixture of many proteins and other biomolecules. However, many tests are now available that use individual, highly-purified allergen components on the solid phase of the assay. The use of allergen components has been referred to as component-resolved diagnostics, or more recently as precision allergy molecular diagnostics, and has helped paved the way for high-throughput multiplex assays in which IgE to several allergen components (and in some cases extracts) can be measured simultaneously in a single assay. Most IgE multiplex assays use microarray technology (i.e., microchip-based), though multiplex bead-based platforms and other technologies have also been developed.

Fig.1 Schematic representation of a multiplex immunoassay, generally used for allergy diagnosis.¹

Application

Antigen Microarray in Allergens Diagnosis

Antigen microarrays offer a high-throughput platform for allergen diagnosis by simultaneously detecting multiple allergen-specific IgE antibodies in patient samples. They enable comprehensive profiling of allergic sensitivities, helping in identifying specific allergens that cause responses. This technology allows for personalized allergy testing, facilitating more accurate diagnosis and tailored treatment plans.

The primary advantage of adopting a multiplexed platform, such as a microarray, for component-resolved testing is that specific IgE to potentially thousands of allergens can be assayed in parallel with a small amount of serum. Because a traditional solid-phase assay typically requires the order of 10 to 100 mL per test, this might already begin to become practically limiting for profiling more than 100 tests in some patients (e.g., children). By using a microarray-based assay, that same volume is more than sufficient to assay more than 1000 targets with controls and replicates. At the same time, much less allergen (approximately 1000-fold) is required per assay, and this is also a significant consideration because some allergens are difficult to express, purify, or both.

Table 1. Applications of microarray-based molecular allergen assays.

Our Service

Services at Creative Biolabs

Antigen microarray is a detection method with the advantages of high throughput. For common allergens detection, this advantage is of great importance. Focusing on autoantibodies and relevant research over the years, Creative Biolabs has accumulated extensive experience from practice. We have also established a systematical technology platform providing Antigen Microarray Detection for Common Allergens from food, dust, pets, mites, insects, pollen to help our customers accelerate their projects. With rich experience and strong foundations, we are confident in offering customer-satisfied services.

Highlight

• A tiny quantity of serum.
• Excellent clinical specificity.
• Parallel statistical power of assaying
• Ability to simultaneously use multiple readout parameters.
• Higher target resolution and sensitivity.

FAQs

• Q1: How do you ensure that test results are clinically relevant?
  A: Yeah, it's important to ensure that the results obtained from the chip testing have clinical relevance. Our scientists perform test analysis by correlating chip data with patient symptoms and medical history to achieve good clinical correlation.
• Q2: What is the difference between forward-phase and reversed-phase protein microarrays?
  A: Forward-phase arrays immobilize antibodies on a solid support, allowing detection of antigen binding. Reverse-phase arrays immobilize bait molecules such as serum protein, a cellular lysate, or subcellular protein fraction, enabling the detection of antibody binding. Forward-phase arrays are suitable for epitope mapping and antibody profiling, while reversed-phase arrays are used for studying protein expression levels and serum antibody profiling.

Resource

If you are interested in antigen microarray detection, or you have any other questions about our autoantibodies-related services, please feel free to contact us for more information.

Reference

1. Tuppo, Lisa, et al. "Detection of allergenic proteins in foodstuffs: Advantages of the innovative multiplex allergen microarray-based immunoassay compared to conventional methods." Foods 11.6 (2022): 878.

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