Q: What is the purpose of the Human CM1 Autoantibody ELISA Kit?
A: An ELISA kit like this is designed to qualitatively determine the presence of CM1 Autoantibodies in human plasma or serum. This can be essential for research or diagnostic purposes related to specific immune responses or conditions.
Q: How does the ELISA kit work?
A: ELISA, or Enzyme-Linked Immunosorbent Assay, kits involve a plate coated with an antigen related to the antibody of interest. When a sample containing the antibody is added, it binds to the antigen. Subsequent steps involve washing, adding a secondary antibody conjugated with an enzyme, and a substrate that the enzyme acts on to produce a measurable signal.
Q: Are there any critical considerations or limitations when using the ELISA kit?
A: Yes, it's crucial to follow the kit's protocol precisely to ensure accurate results. Factors such as sample preparation, incubation times, and temperatures can significantly affect the outcome. Additionally, the interpretation of results should consider the possibility of cross-reactivity and the specificity and sensitivity of the kit for the autoantibodies of interest.
Q: How should the ELISA kit be stored?
A: Storage instructions typically include short-term storage temperatures (e.g., 4°C) and guidelines for longer-term storage, which might require freezing. Adhering to these guidelines ensures the kit's components remain stable and effective.
Datasheet
Detection of CM1 in normal individuals and cancer patients by ELISA.